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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Trypanosoma cruzi: Role of delta-Amastin on Extracellular Amastigote Cell Invasion and Differentiation

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Author(s):
Cruz, Mario C. [1] ; Souza-Melo, Normanda [2] ; da Silva, Claudio Vieira [3] ; DaRocha, Wanderson Duarte [2] ; Bahia, Diana [1] ; Araujo, Patricia R. [4] ; Teixeira, Santuza R. [4] ; Mortara, Renato A. [1]
Total Authors: 8
Affiliation:
[1] Escola Paulista Med UNIFESP, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
[2] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-80060000 Curitiba, Parana - Brazil
[3] Univ Fed Uberlandia, Disciplina Imunol, Inst Ciencias Biomed, BR-38400 Uberlandia, MG - Brazil
[4] Univ Fed Minas Gerais, Dept Bioquim & Imunol, Belo Horizonte, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 7, n. 12 DEC 18 2012.
Web of Science Citations: 19
Abstract

Trypanosoma cruzi is a protozoan parasite that comprises different phylogenetic groups and is the causative agent of Chagas' disease. Different T. cruzi strains present differences in infectivity in in vitro and in vivo experimental models, which are likely related to the expression of different virulence factors. Amastin is a surface glycoprotein abundantly expressed on the intracellular mammalian amastigote form of the parasite. In this study, we showed that a highly infective strain (G strain) of extracellular amastigote (EA) invasive forms expressed reduced RNA levels of amastin compared to a less infective strain (CL). The treatment of HeLa cells with recombinant delta-amastin reduced infectivity of EA forms. However, the ectopic expression of delta-amastin accelerated amastigote differentiation into trypomastigotes. Corroborating the virulence behavior in association with amastin expression, the EAs overexpressing amastin were precociously and robustly observed in the liver of susceptible mouse strains (A/JUnib), whereas parasitemia was never detected in in vivo assays. This is the first report on the regulatory role of amastin in the course of both in vitro and in vivo T. cruzi infection. Citation: Cruz MC, Souza- Melo N, da Silva CV, DaRocha WD, Bahia D, et al. (2012) Trypanosoma cruzi: Role of delta-Amastin on Extracellular Amastigote Cell Invasion and Differentiation. PLoS ONE 7(12): e51804. doi:10.1371/journal.pone.0051804 (AU)

FAPESP's process: 06/61450-0 - Molecular studies on Trypanosoma cruzi and its interaction with cells and factors from the host in vitro and in vivo
Grantee:José Franco da Silveira Filho
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/51475-3 - Molecular and cellular biology of the parasitism by Trypanosoma cruzi
Grantee:José Franco da Silveira Filho
Support type: Research Projects - Thematic Grants