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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of artificial saliva in biofilm formation of Candida albicans in vitro

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Author(s):
Silva, Michelle Peneluppi [1] ; Chibebe Junior, Jose [1] ; Jorjao, Adeline Lacerda [1] ; da Silva Machado, Ana Karina [1] ; de Oliveira, Luciane Dias [1] ; Junqueira, Juliana Campos [1] ; Cardoso Jorge, Antonio Olavo [1]
Total Authors: 7
Affiliation:
[1] UNESP Univ Estadual Paulista, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Brazilian Oral Research; v. 26, n. 1, p. 24-28, JAN-FEB 2012.
Web of Science Citations: 8
Abstract

Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 degrees C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 10(6) cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 degrees C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 degrees C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p <= 0.05). (AU)

FAPESP's process: 10/14434-4 - Photodynamic therapy in spores of Bacillus atrophaeus and Bacillus subtilis: studies with laser, led, methylene blue, Rose Bengal and malachite green
Grantee:Michelle Peneluppi Silva
Support type: Scholarships in Brazil - Master