Low-level laser therapy in experimental model of collagenase-induced tendinitis in rats: effects in acute and chronic inflammatory phases

Author(s)

Casalechi, Heliodora Leao ^[1] ; Pinto Leal-Junior, Ernesto Cesar ^{[1, 2]} ; Xavier, Murilo ^{[3, 4]} ; Silva, Jr., Jose Antonio ^[1] ; de Tarso, Paulo ; de Carvalho, Camillo ^{[1, 2]} ; Aimbire, Flavio ^[5] ; Albertini, Regiane ^{[1, 2]}

Affiliation

^[1] Nove de Julho Univ UNINOVE, Post Grad Program Rehabil Sci, BR-01504001 Sao Paulo - Brazil

^[2] Nove de Julho Univ UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo - Brazil

^[3] Univ Camilo Castelo Branco, Ctr Biomed Engn, Sao Jose Dos Campos, SP - Brazil

^[4] Vale Jequitinhonha & Mucuri Fed Univ, Dept Phys Therapy, Diamantina, MG - Brazil

^[5] Fed Univ Sao Paulo UNIFESP, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 28, n. 3, p. 989-995, MAY 2013.
• Web of Science Citations: 39

Abstract

A variety of treatments for tendinopathies is currently used or has been trialed. However, in fact, there is a remarkably little evidence that any conventional therapies are effective. In the last years, low-level laser therapy (LLLT) has been showing interesting results in inflammatory modulation in different musculoskeletal disorders, but the optimal parameters and mechanisms behind these effects are not fully understood. The aim of this study is to investigate if the LLLT modulates the acute and chronic phase of collagenase-induced tendinitis in rat by interfering in mRNA expression for matrix metalloproteinases (MMP13 and MMP1), vascular endothelial growth factor (VEGF), and anti-inflammatory mediator (interleukin (IL)-10). For such, tendinitis was induced by collagenase injection in male Wistar rats. Animals were treated with LLLT (780 nm, potency of 22 mW, 107 mW/cm(2), energy density of 7.5 J/cm(2), and energy delivered of 1.54 J) with different number of treatments in accordance with the inflammatory phase analyzed. LLLT was able to modulate mRNA gene expression of IL-10, VGEF, MMP1, and MMP13 both in acute than in chronic inflammatory phase (p < 0.05). Our results suggest that LLLT with parameters employed in the present study was able to modulate IL-10, VEGF, MMP1, and MMP13 mRNA gene expression both in acute than in chronic tendon inflammation. However, further studies are needed to establish optimal parameters for LLLT. (AU)

• FAPESP's process: 12/02087-3 - Modulation of anti-inflammatory factors in a model of traumatic injury in rat Achilles tendon
• Grantee: Heliodora Leão Casalechi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/05890-9 - Modulation of anti-inflammatory factors in the model foterapia traumatic Achilles tendon in rats
• Grantee: Regiane Albertini
• Support Opportunities: Regular Research Grants