CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

Author(s)

Carla Renata Sipert ^[1] ; Ana Carolina de Faria Morandini ^[1] ; Karin Cristina da Silva Modena ^[2] ; Thiago Jose Dionisio ^[1] ; Maria Aparecida Andrade Moreira Machado ^[3] ; Sandra Helena Penha de Oliveira ^[4] ; Ana Paula Campanelli ^[1] ; Carlos Ferreira Santos ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, BR-17012901 Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Community Hlth, BR-17012901 Bauru, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Dept Basic Sci, Aracatuba Sch Dent, Aracatuba, SP - Brazil

• Document type: Journal article
• Source: Journal of Applied Oral Science; v. 21, n. 2, p. 99-105, 2013-04-00.

Abstract

Objective: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). Material and Methods: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0 – 10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. Results: Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF. Conclusion: These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation. (AU)

• FAPESP's process: 05/60167-0 - Expression of procollagen type 1, MIP-1± alpha and SDF-1 alpha by human fibroblasts stimulated pulp lipoteichoic acid from Streptococcus mutans
• Grantee: Carlos Ferreira dos Santos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 07/00306-1 - Expression of procollagen type I, MIP-1alpha and SDF-1alpha by human gingival fibroblasts and dental pulp stimulated by Enterococcus faecalis lipoteichoic acid and lipopolysaccharide from Escherichia coli and Porphyromonas gingivalis
• Grantee: Carla Renata Sipert
• Support Opportunities: Scholarships in Brazil - Doctorate