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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bioactivity and cytotoxicity of glass and glass-ceramics based on the 3CaO center dot P2O5-SiO2-MgO system

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Daguano, Juliana K. M. F. [1] ; Rogero, Sizue O. [2] ; Crovace, Murilo C. [3] ; Peitl, Oscar [3] ; Strecker, Kurt [4] ; dos Santos, Claudinei [1, 5]
Total Authors: 6
[1] Univ Sao Paulo, Dept Engn Mat, Escola Engn Lorena, Lorena, SP - Brazil
[2] Inst Pesquisas Energet & Nucl, Sao Paulo - Brazil
[3] Univ Fed Sao Carlos, Lab Mat Vitreos, Dept Mat Engn, BR-13560 Sao Carlos, SP - Brazil
[4] Univ Fed Sao Joao del Rei, Sao Joao Del Rei, MG - Brazil
[5] UNIFOA, Ctr Univ Volta Redonda, Volta Redonda, RJ - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 24, n. 9, p. 2171-2180, SEP 2013.
Web of Science Citations: 9

The mechanical strength of bioactive glasses can be improved by controlled crystallization, turning its use as bulk bone implants viable. However, crystallization may affect the bioactivity of the material. The aim of this study was to develop glass-ceramics of the nominal composition (wt%) 52.75(3CaO center dot P2O5)-30SiO(2)-17.25MgO, with different crystallized fractions and to evaluate their in vitro cytotoxicity and bioactivity. Specimens were heat-treated at 700, 775 and 975 A degrees C, for 4 h. The major crystalline phase identified was whitlockite, an Mg-substituted tricalcium phosphate. The evaluation of the cytotoxicity was carried out by the neutral red uptake methodology. Ionic exchanges with the simulated body fluid SBF-K9 acellular solution during the in vitro bioactivity tests highlight the differences in terms of chemical reactivity between the glass and the glass-ceramics. The effect of crystallinity on the rates of hydroxycarbonate apatite (HCA) formation was followed by Fourier transformed infrared spectroscopy. Although all glass-ceramics can be considered bioactive, the glass-ceramic heat-treated at 775 A degrees C (V775-4) presented the most interesting result, because the onset for HCA formation is at about 24 h and after 7 days the HCA layer dominates completely the spectrum. This occurs probably due to the presence of the whitlockite phase (3(Ca,Mg)O center dot P2O5). All samples were considered not cytotoxic. (AU)

FAPESP's process: 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass
Grantee:Edgar Dutra Zanotto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC