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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanoparticles of Lyotropic Liquid Crystals: A Novel Strategy for the Topical Delivery of a Chlorin Derivative for Photodynamic Therapy of Skin Cancer

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Petrilli, Raquel [1] ; Praca, Fabiola S. G. [1] ; Carollo, Aline Regina H. [1] ; Medina, Wanessa S. G. [1] ; de Oliveira, Kleber Thiago [2] ; Fantini, Marcia C. A. [3] ; Neves, Maria da Graca P. M. S. [4, 5] ; Cavaleiro, Jose A. S. [4, 5] ; Serra, Osvaldo A. [6] ; Iamamoto, Yassuko [6] ; Bentley, Maria Vitoria L. B. [1]
Total Authors: 11
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Quim, BR-13560 Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Inst Fis, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Univ Aveiro, Dept Chem, Aveiro - Portugal
[5] Univ Aveiro, QOPNA, Aveiro - Portugal
[6] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: CURRENT NANOSCIENCE; v. 9, n. 4, p. 434-441, AUG 2013.
Web of Science Citations: 15

Nanoparticles of lyotropic liquid crystals loaded with a new photosensitizer (a chlorin derivative) were developed for use in photodynamic therapy (PDT). These systems were characterized by spectrofluorimetric, dynamic light scattering, and small angle X-ray diffraction (SAXRD) analyses. In vitro and in vivo penetration studies in animal models were performed using animal model membranes. The systems had a particle size of 161 +/- 4 nm and a polydispersity index of 0.175 +/- 0.027. Furthermore, SAXRD studies demonstrated that the preparations remained in the liquid crystalline phase type hexagonal after drug loading. The encapsulation rate was higher than 50%, and cell viability studies revealed that the nanodispersion is not harmful for L929 skin cells. In vitro and in vivo penetration studies confirmed that the nanodispersion of hexagonal phase enabled a higher drug skin uptake compared to the control. Additionally, a fluorescence microscopy study demonstrated a higher biodistribution of the chlorin derivative in the skin layers of hairless mice compared to the control. Taken together, the results show the potential of this nanodispersion for the delivery of the photosensitizer into the skin, which is a crucial condition for successful topical PDT. (AU)

FAPESP's process: 09/00009-2 - Synthesis and characterization of new porphyrinic and related macrocycles systems for the study of catalytic and photosensitizers properties
Grantee:Yassuko Iamamoto
Support type: Regular Research Grants
FAPESP's process: 08/06619-4 - Synthesis of new photosensitizers with potential aplication in photodynamic therapy
Grantee:Kleber Thiago de Oliveira
Support type: Research Grants - Young Investigators Grants