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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Serotonergic neurons in the nucleus raphe obscurus are not involved in the ventilatory and thermoregulatory responses to hypoxia in adult rats

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da Silva, Glauber S. F. [1, 2] ; Giusti, Humberto [1] ; Castro, Olagide W. [1] ; Garcia-Cairasco, Norberto [1] ; Gargaglioni, Luciane H. [3] ; Branco, Luiz G. S. [2] ; Glass, Mogens L. [1]
Total Authors: 7
[1] Univ Sao Paulo, FMRP, Dept Physiol, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, FORP, Dept Morphol Physiol & Pathol, BR-14040904 Ribeirao Preto, SP - Brazil
[3] Sao Paulo State Univ, UNESP, FCAV, Dept Anim Morphol & Physiol, Jaboticabal, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Respiratory Physiology & Neurobiology; v. 187, n. 2, p. 139-148, JUN 15 2013.
Web of Science Citations: 7

The medullary raphe is an important component of the central respiratory network, playing a key role in CO2 central chemoreception. However, its participation in hypoxic ventilatory responses is less understood. In the present study, we assessed the role of nucleus raphe obscurus (ROb), and specifically 5-HT neurons confined in the ROb, on ventilatory and thermoregulatory responses to hypoxia. Chemical lesions of the ROb were performed using either ibotenic acid (non-specific lesion; control animals received PBS) or anti-SERT-SAP (5-HT specific lesion; control animals received IgG-SAP). Ventilation (VE; whole body plethysmograph) and body temperature (Tb; data loggers) were measured during normoxia (21% O-2, N-2 balance) and hypoxia exposure (7% O-2, N-2 balance, 1 h) in conscious adult rats. Ibotenic acid or antiSERT-SAP-induced lesions did not affect baseline values of VE and Th. Similarly, both lesion procedures did not alter the ventilatory or thermoregulatory responses to hypoxia. Although evidence in the literature suggests a role of the rostral medullary raphe in hypoxic ventilatory responses, under the present experimental conditions our data indicate that caudal medullary raphe (ROb) and its 5-HT neurons neither participate in the tonic maintenance of breathing nor in the ventilatory and thermal responses to hypoxia. (C) 2013 Elsevier B.V. All rights reserved. (AU)