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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Slimmer or Fertile? Pharmacological Mechanisms Involved in Reduced Sperm Quality and Fertility in Rats Exposed to the Anorexigen Sibutramine

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Author(s):
Borges, Cibele S. [1] ; Missassi, Gabriela [1] ; Pacini, Enio S. A. [2] ; Kiguti, Luiz Ricardo A. [2] ; Sanabria, Marciana [1] ; Silva, Raquel F. [1] ; Banzato, Thais P. [1] ; Perobelli, Juliana E. [1] ; Pupo, Andre S. [2] ; Kempinas, Wilma G. [1]
Total Authors: 10
Affiliation:
[1] UNESP Univ Estadual Paulista, Inst Biosci, Dept Morphol, Botucatu, SP - Brazil
[2] UNESP Univ Estadual Paulista, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 8, n. 6 JUN 12 2013.
Web of Science Citations: 11
Abstract

Sperm acquire motility and fertility capacity during epididymal transit, under the control of androgens and sympathetic innervations. It is already known that the acceleration of epididymal sperm transit time can lead to lower sperm quality. In a previous work we showed that rats exposed to the anorexigen sibutramine, a non-selective serotonin-norepinephrine reuptake inhibitor, presented faster sperm transit time, lower epididymal sperm reserves and potentiation of the tension of epididymal duct to norepinephrine exposed acutely in vitro to sibutramine. In the present work we aimed to further investigate pharmacological mechanisms involved in these alterations and the impact on rat sperm quality. For this, adult male Wistar rats were treated with sibutramine (10 mg/kg/day) or vehicle for 30 days. Sibutramine decreased final body, seminal vesicle, ventral prostate and epididymal weights, as well as sperm transit time in the epididymal cauda. On the contrary of the in vitro pharmacological assays, in which sibutramine was added directly to the bath containing strips of distal epididymal cauda, the ductal tension was not altered after in vivo sub-chronic exposure to sibutramine. However, there is pharmacological evidence that the endogenous epididymal norepinephrine reserves were reduced in these animals. It was also shown that the decrease in prostate weight can be related to increased tension developed of the gland, due to sibutramine sympathomimetic effects. In addition, our results showed reduced sperm quality after in utero artificial insemination, a more sensitive procedure to assess fertility in rodents. The epididymal norepinephrine depletion exerted by sibutramine, associated with decreases in sperm transit time, quantity and quality, leading to reduced fertility in this experimental model, reinforces the concerns about the possible impact on fertility of man taking sibutramine as well as other non-selective serotonin-norepinephrine reuptake inhibitors, especially considering the lower reproductive efficiency of humans compared to males of other species. (AU)

FAPESP's process: 10/13948-4 - Effects of the treatment with sibutramine on sperm quality of Male Wistar rats
Grantee:Cibele dos Santos Borges
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/11840-4 - Effects of sibutramine on reproductive parameters of male rats
Grantee:Gabriela Missassi
Support type: Scholarships in Brazil - Scientific Initiation