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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Vitro Susceptibility of Environmental Isolates of Exophiala dermatitidis to Five Antifungal Drugs

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Miranda Duarte, Ana Paula [1] ; Pagnocca, Fernando Carlos [2, 1] ; Baron, Noemi Carla [1] ; Carvalho Melhem, Marcia de Souza [3] ; Palmeira, Gislene Aparecida [3] ; de Angelis, Dejanira de Franceschi [2] ; Attili-Angelis, Derlene [2, 1, 4]
Total Authors: 7
[1] UNESP Sao Paulo State Univ, IB, Ctr Study Social Insects, BR-13506900 Rio Claro, SP - Brazil
[2] UNESP Sao Paulo State Univ, IB, Dept Biochem & Microbiol, BR-13506900 Rio Claro, SP - Brazil
[3] Adolfo Lutz Publ Hlth Reference Lab, Rio Claro, SP - Brazil
[4] Univ Estadual Campinas, CPQBA, Div Microbial Resources, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Mycopathologia; v. 175, n. 5-6, SI, p. 455-461, JUN 2013.
Web of Science Citations: 9

Several dematiaceous fungi frequently isolated from nature are involved in cases of superficial lesions to lethal cerebral infections. Antifungal susceptibility data on environmental and clinical isolates are still sparse despite the advances in testing methods. The objective of this study was to examine the activities of 5-flucytosine, amphotericin B, itraconazole, voriconazole and terbinafine against environmental isolates of Exophiala strains by minimum inhibition concentration (MIC) determination. The strains were obtained from hydrocarbon-contaminated soil, ant cuticle and fungal pellets from the infrabuccal pocket of attine gynes. Broth microdilution assay using M38-A2 reference methodology for the five antifungal drugs and DNA sequencing for fungal identification were applied. Terbinafine was the most active drug against the tested strains. It was observed that amphotericin B was less effective, notably against Exophiala spinifera, also studied. High MICs of 5-flucytosine against Exophiala dermatitidis occurred. This finding highlights the relevance of studies on the antifungal resistance of these potential opportunistic species. Our results also contribute to a future improvement of the standard methods to access the drug efficacy currently applied to black fungi. (AU)