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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crystallization and preliminary X-ray diffraction analysis of a new xyloglucanase from Xanthomonas campestris pv. campestris

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Author(s):
de Araujo, Evandro Ares [1] ; Tomazini, Jr., Atilio [1] ; Seiki Kadowaki, Marco Antonio [1] ; Murakami, Mario Tyago [2] ; Polikarpov, Igor [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
[2] CNPEM, Lab Nacl Biociencias, BR-13083100 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 69, n. 6, p. 676-678, JUN 2013.
Web of Science Citations: 0
Abstract

Xyloglucanases (Xghs) are important enzymes involved in xyloglucan modification and degradation. Xanthomonas campestris pv. campestris (Xcc) is a phytopathogenic bacterium which produces a large number of glycosyl hydrolases (GH), but has only one family 74 GH (Xcc-Xgh). This enzyme was overexpressed in Escherichia coli, purified and crystallized. Diffraction data sets were collected for the native enzyme and its complex with glucose to maximum resolutions of 2.0 and 2.1 angstrom, respectively. The data were indexed in a hexagonal crystal system with unit-cell parameters a = b = 153.4, c = 84.9 angstrom. As indicated by molecular-replacement solution, the crystals belonged to space group P6(1). (AU)

FAPESP's process: 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery
Grantee:Igor Polikarpov
Support type: Regular Research Grants
FAPESP's process: 07/08706-9 - Molecular and structural studies of Cellulitic complex enzymes of fungi Trichoderma harzianum with potencial of enzymatic transformation of biomass
Grantee:Viviane Isabel Serpa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis
Grantee:Igor Polikarpov
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 09/05349-6 - Expression and purification of cellobiohydrolase I from the filamentous fungus Trichoderma harzianum
Grantee:Bruno Luan Soares Paula de Mello
Support type: Scholarships in Brazil - Scientific Initiation