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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Leptin Modulates Norepinephrine-Mediated Melatonin Synthesis in Cultured Rat Pineal Gland

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Author(s):
Peliciari-Garcia, Rodrigo Antonio [1] ; Andrade-Silva, Jessica [1] ; Cipolla-Neto, Jose [1] ; de Oliveira Carvalho, Carla Roberta [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BIOMED RESEARCH INTERNATIONAL; 2013.
Web of Science Citations: 5
Abstract

Pineal melatonin synthesis can be modulated by many peptides, including insulin. Because melatonin appears to alter leptin synthesis, in this work we aimed to investigate whether leptin would have a role on norepinephrine-(NE-)mediated melatonin synthesis in cultured rat pineal glands. According to our data, cultured rat pineal glands express leptin receptor isoform b (Ob-Rb). Pineal expression of Ob-Rb mRNA was also observed in vivo. Administration of leptin (1 nM) associated with NE (1 mu M) reduced melatonin content as well as arylalkylamine-N-acetyl transferase (AANAT) activity and expression in cultured pineal glands. Leptin treatment per se induced the expression of STAT3 in cultured pineal glands, but STAT3 does not participate in the leptin modulation of NE-mediated pineal melatonin synthesis. In addition, the expression of inducible cAMP early repressor (ICER) was further induced by leptin challenge when associated with NE. In conclusion, leptin inhibition of pineal melatonin synthesis appears to be mediated by a reduction in AANAT activity and expression as well as by increased expression of Icer mRNA. Peptidergic signaling within the pineal gland appears to be one of the most important signals which modulates melatonin synthesis; leptin, as a member of this system, is not an exception. (AU)

FAPESP's process: 09/52920-0 - Melatonin and the control of energy metabolism: central and peripheral actions and the circadian timing of the metabolic function
Grantee:José Cipolla Neto
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/04359-8 - Role of noradrenergic synchronized on melatonin synthesis of pineal gland culture: functionals characteristics and action mechanisms
Grantee:Jéssica Andrade da Silva
Support type: Scholarships in Brazil - Master