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Interpopulational variation of secondary metabolites of arnicão (Lychnophora salicifolia Mart., Vernonieae, Asteraceae).

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Author(s):
Dayana Rubio Gouvea
Total Authors: 1
Document type: Master's Dissertation
Press: Ribeirão Preto.
Institution: Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC)
Defense date:
Examining board members:
Norberto Peporine Lopes; Carmen Lúcia Cardoso; Maria Fatima das Graças Fernandes da Silva
Advisor: Norberto Peporine Lopes
Abstract

About 10% of the world flora is represented by the Asteraceae family, which has been extensively studied since it is one of the largest families of Angiospermae, characterized not only by morphological and taxonomic diversity but also the wealth of secondary metabolites produced by the species studied of this family. Lychnophora salicifolia Mart. is the most widely distributed species of the genus (occurring in the Serra do Espinhaço in Bahia, Minas Gerais and in the southeast of Goiás) and also is the most polymorphic. Its popular use is due to the anti-inflammatory and analgesic activity. In this work, we studied the variation of polar metabolites of this species using the dereplication of the crude extract (methanol/water obtained from the leaves) by the HPLC-DAD-MS analysis, which led us to identify the constituents of this mixture by analyzing the UV spectra and MS data. Thus it was possible to identify 20 substances: protocatechuic acid; 3-O-E-caffeoylquinic acid; 3-O-p-coumaroylquinic acid; 4-O-E-caffeoylquinic acid; 5-O-E-caffeoylquinic acid; 4-O-p-coumaroylquinic acid; 5-O-p-coumaroylquinic acid; 5-O-feruloylquinic acid; vicenin-2 (6,8-di-C--glucosylapigenin); 3,4-di-O-E-caffeoylquinic acid, 3,5-di-O-E-caffeoylquinic acid; 3-O-coumaroyl-5-O-p-caffeoylquinic acid; 3-O-caffeoyl acid, 5-O-p-coumaroylquinic acid; 4,5-di-O-E-caffeoylquinic acid, 4-feruloyl-5-O-E-caffeoylquinic acid; 3,4-di-O-p-coumaroylquinic acid; 3,5-di-O-p-coumaroylquinic acid; 4,5-di-O-p-coumaroylquinic acid; lychnofolic acid and 2-O-acetyl lychnofolic acid. To the validation of analytical methodology were used one substance belonging to each class of compounds: 5-O-E-caffeoylquinic acid, vicenin-2 and 2-O-acetyl lychnofolic acid. So, the validation of analytical method ensured the reliability and credibility of the results of the method for purposes of this study, covering the concentration range required for analysis of population variation and presents appropriates values of detection and quantification limits, precision and recovery. For intrapopulation analysis, there was a quantitative variability between individuals of the same population, but on average the individuals of the same population are similar chemically. In relation to the study of interpopulation variation, we could see quantitative and qualitative differences between populations collected from different regions of Brazil, and from the statistical analysis, we found that there is a correlation between geographic isolation and decrease of the similarity of the polar metabolites profile between plants of different populations. This indicated that the pattern found for the concentration of metabolites is influenced by the geographical distribution of samples. However, it is not possible to determine the location of the samples only by the metabolic variables studied. Thus, the presence and concentration of metabolites are influenced by the geographical distribution of samples. (AU)