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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma

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Author(s):
Fonseca, Felipe Paiva [1] ; Benevenuto de Andrade, Bruno Augusto [1] ; Carrinho Ayrosa Rangel, Ana Lucia ; Della Coletta, Ricardo [1] ; Lopes, Marcio Ajudarte [1] ; de Almeida, Oslei Paes [1] ; Vargas, Pablo Agustin [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 117, n. 1, p. 81-88, JAN 2014.
Web of Science Citations: 20
Abstract

Objective. To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Study design. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. Results. The 1.0-mm TMA presented lower results than 2.0-and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Conclusions. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study. (AU)

FAPESP's process: 12/07519-9 - SEMAPHORINS AND NEUROPILINS IN SALIVARY GLAND TUMORS
Grantee:Felipe Paiva Fonseca
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/53839-2 - Creation of a Digital Pathology Laboratory using a histological slidescanner
Grantee:Oslei Paes de Almeida
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 12/10781-7 - Analysis of the expression of cytokeratins, angiogenic and proliferative patterns, and DNA content of salivary gland tumors
Grantee:Pablo Agustin Vargas
Support Opportunities: Regular Research Grants