Synergistic effects of agroindustrial wastes on simultaneous production of protease and alpha-amylase under solid state fermentation using a simplex centroid mixture design

Author(s)

Soares de Castro, Ruann Janser ^[1] ; Sato, Helia Harumi ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Food Sci, Sch Food Engn, Campinas, SP - Brazil

• Document type: Journal article
• Source: INDUSTRIAL CROPS AND PRODUCTS; v. 49, p. 813-821, AUG 2013.
• Web of Science Citations: 17

Abstract

Wheat bran, soybean meal and cottonseed meal, alone or in combinations, were used as the substrates for simultaneous production of protease and alpha-amylase by Aspergillus oryzae under solid state fermentation using a simplex centroid mixture design. The substrates with highest water absorption index and more homogeneous granulometric distribution have positively influenced on enzymes production. Synergistic effects of agroindustrial wastes observed on the production of protease and alpha-amylase resulted in increases ranged from 6.3 to 106.1% at 24h fermentation. Mixtures contained wheat bran (1/2) and soybean meal (1/2) or wheat bran (1/2) and cottonseed meal (1/2) may be added to improve the enzymes production in shorter time fermentation. However, at 48 and 72 h fermentation, the most of the interaction effects observed were antagonistic and the highest protease and alpha-amylase activities were obtained wheat bran as the substrate. This innovative process showed maximization of alpha-amylase and proteases production when mixtures were used compared to the isolated substrates and can be extended to other enzymes groups for obtaining multi-enzyme complex. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/10429-9 - Production and application of proteases from Aspergillus oryzae to obtain peptides with biological activity
• Grantee: Hélia Harumi Sato
• Support Opportunities: Regular Research Grants