Effects of different intensities of physical exercise on insulin sensitivity and protein kinase B/Akt activity in skeletal muscle of obese mice

Author(s)

Rodolfo Marinho ^[1] ; Leandro Pereira de Moura ^[2] ; Bárbara de Almeida Rodrigues ^[3] ; Luciana Santos Souza Pauli ^[4] ; Adelino Sanchez Ramos da Silva ^[5] ; Eloize Cristina Chiarreotto Ropelle ^[6] ; Claudio Teodoro de Souza ^[7] ; Dennys Esper Corrêa Cintra ^[8] ; Eduardo Rochete Ropelle ^[9] ; José Rodrigo Pauli

Affiliation

^[1] Universidade Estadual Paulista Júlio de Mesquita Filho - Brasil

^[2] Universidade Estadual Paulista Júlio de Mesquita Filho - Brasil

^[3] Universidade Estadual de Campinas - Brasil

^[4] Universidade Estadual de Campinas - Brasil

^[5] Universidade de São Paulo - Brasil

^[6] Universidade Estadual de Campinas - Brasil

^[7] Universidade do Extremo Sul Catarinense - Brasil

^[8] Universidade Estadual de Campinas - Brasil

^[9] Universidade Estadual de Campinas - Brasil

• Document type: Journal article
• Source: Einstein (São Paulo); v. 12, n. 1, p. 82-89, 2014-03-00.

Abstract

Objective : To investigate the effects of different intensities of acute exercise on insulin sensitivity and protein kinase B/Akt activity in skeletal muscle of obese mice. Methods : Swiss mice were randomly divided into four groups, and fed either a standard diet (control group) or high fat diet (obese sedentary group and obese exercise group 1 and 2) for 12 weeks. Two different exercise protocols were used: swimming for 1 hour with or without an overload of 5% body weight. The insulin tolerance test was performed to estimate whole-body sensitivity. Western blot technique was used to determine protein levels of protein kinase B/Akt and phosphorylation by protein Kinase B/Akt in mice skeletal muscle. Results : A single bout of exercise inhibited the high fat diet-induced insulin resistance. There was increase in phosphorylation by protein kinase B/Akt serine, improve in insulin signaling and reduce of fasting glucose in mice that swam for 1 hour without overload and mice that swan for 1 hour with overload of 5%. However, no significant differences were seen between exercised groups. Conclusion : Regardless of intensity, aerobic exercise was able to improve insulin sensitivity and phosphorylation by protein kinase B/Ak, and proved to be a good form of treatment and prevention of type 2 diabetes. (AU)

• FAPESP's process: 12/01750-0 - Effects of aerobic physical training on inflammatory and apoptotic process in hypothalamic neurons of hungry control in high-fat diet-induced obese mice.
• Grantee: Rodolfo Marinho
• Support Opportunities: Scholarships in Brazil - Doctorate