Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin

Author(s)

Rodrigues, Anderson Messias ^{[1, 2]} ; de Hoog, G. Sybren ^[1] ; de Camargo, Zoilo Pires ^[2]

Affiliation

^[1] CBS KNAW Fungal Biodivers Ctr, Utrecht - Netherlands

^[2] Univ Fed Sao Paulo, Cellular Biol Div, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

• Document type: Journal article
• Source: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 78, n. 4, p. 383-387, APR 2014.
• Web of Science Citations: 31

Abstract

Sporotrichosis is one of the most common subcutaneous mycosis in Latin America and is caused by 4 pathogenic thermodimorphic fungi in the genus Sporothrix. From both therapeutic and epidemiological perspectives, it is essential to identify the causative agents down to the species level. Traditional parameters may overlap among closely related species, and we propose polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as an alternative approach. In the present study, the calmodulin gene was amplified and digested with Hhal to yield 5 different electrophoretic patterns representing all medically important Sporothrix species: Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, and Sporothrix luriei. The PCR-RFLP protocol described here is a simple and inexpensive method and is highly suitable for accurate routine genotyping of relevant Sporothrix species. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 11/07350-1 - Biology of Sporothrix schenckii complex: Analysis of molecular and proteomics markers, and the role of antigenic and antifungal molecules in sporotrichosis
• Grantee: Anderson Messias Rodrigues
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/54024-2 - Molecular biology and proteomics of medically interesting fungi: Paracoccidioides brasiliensis and Sporothrix schenckii
• Grantee: Zoilo Pires de Camargo
• Support Opportunities: Research Projects - Thematic Grants