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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phylogeny of culturable cyanobacteria from Brazilian mangroves

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Author(s):
Pamplona Silva, Caroline Souza [1] ; Genuario, Diego Bonaldo [1] ; Marcal Vieira Vaz, Marcelo Gomes [1] ; Fiore, Marli Fatima [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Lab Mol Ecol Cyanobacteria, BR-13400970 Piracicaba, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Systematic and Applied Microbiology; v. 37, n. 2, p. 100-112, MAR 2014.
Web of Science Citations: 29
Abstract

The cyanobacterial community from Brazilian mangrove ecosystems was examined using a culture-dependent method. Fifty cyanobacterial strains were isolated from soil, water and periphytic samples collected from Cardoso Island and Bertioga mangroves using specific cyanobacterial culture media. Unicellular, homocytous and heterocytous morphotypes were recovered, representing five orders, seven families and eight genera (Synechococcus, Cyanobium, Cyanobacterium, Chlorogloea, Leptolyngbya, Phormidium, Nostoc and Microchaete). All of these novel mangrove strains had their 16S rRNA gene sequenced and BLAST analysis revealed sequence identities ranging from 92.5 to 99.7% when they were compared with other strains available in GenBank. The results showed a high variability of the 16S rRNA gene sequences among the genotypes that was not associated with the morphologies observed. Phylogenetic analyses showed several branches formed exclusively by some of these novel 16S rRNA gene sequences. BLAST and phylogeny analyses allowed for the identification of Nodosilinea and Oxynema strains, genera already known to exhibit poor morphological diacritic traits. In addition, several Nostoc and Leptolyngbya morphotypes of the mangrove strains may represent new generic entities, as they were distantly affiliated with true genera clades. The presence of non-ribosomal peptide synthetase, polyketide synthase, microcystin and saxitoxin genes were detected in 20.5%, 100%, 37.5% and 33.3%, respectively, of the 44 tested isolates. A total of 134 organic extracts obtained from 44 strains were tested against microorganisms, and 26% of the extracts showed some antimicrobial activity. This is the first polyphasic study of cultured cyanobacteria from Brazilian mangrove ecosystems using morphological, genetic and biological approaches. (C) 2014 Elsevier GmbH. All rights reserved. (AU)

FAPESP's process: 04/13910-6 - Biodiversity and functional activities of microorganisms from mangrove of the State of São Paulo
Grantee:Itamar Soares de Melo
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 06/01671-2 - Molecular characterization of cyanobacteria strains isolated from São Paulo State mangrove regions
Grantee:Caroline Souza Pamplona da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/18732-0 - Bioactive natural products from marine cyanobacteria
Grantee:Marcelo Gomes Marçal Vieira Vaz
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/00321-3 - Diversity and functional activity of cyanobacteria isolated from south Shetlands archipelago, Antarctic continent
Grantee:Diego Bonaldo Genuário
Support type: Scholarships in Brazil - Doctorate