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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HMGB1 Localization during Experimental Periodontitis

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Boas Nogueira, Andressa Vilas [1] ; Chaves de Souza, Joao Antonio [1] ; de Molon, Rafael Scaf [1] ; Mariano Pereira, Elyne da Silva [1] ; de Aquino, Sabrina Garcia [1] ; Giannobile, William V. [2] ; Cirelli, Joni Augusto [1]
Total Authors: 7
[1] Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP - Brazil
[2] Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 - USA
Total Affiliations: 2
Document type: Journal article
Source: Mediators of Inflammation; 2014.
Web of Science Citations: 13

Aim. This study sought to investigate the in vitro expression profile of high mobility group box 1 (HMGB1) in murine periodontal ligament fibroblasts (mPDL) stimulated with LPS or IL-1 beta and in vivo during ligature-or LPS-induced periodontitis in rats. Material and Methods. For the in vivo study, 36 rats were divided into experimental and control groups, and biopsies were harvested at 7-30 d following disease induction. Bone loss and inflammation were evaluated. HMGB1 expression was assessed by immunohistochemistry, qPCR, and Western blot. Results. Significant increases in mPDL HMGB1 mRNA occurred at 4, 8, and 12 h with protein expression elevated by 24 h. HMGB1 mRNA expression in gingival tissues was significantly increased at 15 d in the LPS-PD model and at 7 and 15 d in the ligature model. Immunohistochemical staining revealed a significant increase in the number of HMGB1-positive cells during the experimental periods. Conclusion. The results show that PDL cells produce HMGB1, which is increased and secreted extracellularly after inflammatory stimuli. In conclusion, this study demonstrates that HMGB1 may be associated with the onset and progression of periodontitis, suggesting that further studies should investigate the potential role of HMGB1 on periodontal tissue destruction. (AU)

FAPESP's process: 10/05957-3 - Evaluation of gene expression profile of HMGB-1 in relevant cells of the periodontium
Grantee:Elyne da Silva Mariano Pereira
Support type: Scholarships in Brazil - Scientific Initiation