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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells

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Vargas, Fernanda da Silveira [1] ; Soares, Diana Gabriela [1] ; Dias Ribeiro, Ana Paula [2] ; Hebling, Josimeri [3] ; De Souza Costa, Carlos Alberto [4]
Total Authors: 5
[1] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Oral Rehabil Program, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil
[2] Univ Brasilia, Sch Dent, Dept Operat Dent, BR-70910900 Brasilia, DF - Brazil
[3] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP - Brazil
[4] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 9

The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol (alpha-T) isomer against the toxicity of hydrogen peroxide (H2O2) on dental pulp cells. The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mM alpha-T for 60 minutes. They were then exposed or not to H2O2 for 30 minutes. In positive and negative control groups, the cells were exposed to culture medium with or without H2O2 (0.018%), respectively. Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests; alpha = 5%). Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control. Cells pretreated with alpha-T at 1, 3, 5, and 10 mM concentrations and exposed to H2O2 had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively. These values were significantly lower than those observed in the positive control, thereby showing a protective effect of alpha-T against the H2O2 toxicity. Overall, the vitamin E alpha-T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H2O2. The most effective cell protection was provided by 5 and 10 mM concentrations of alpha-T. (AU)

FAPESP's process: 11/15366-5 - Effects of antioxidant agents (alpha-tocopherol and Trolox) treatment on the trans-enamel and trans-dentinal toxicity of a 35% hydrogen peroxide gel applied on odontoblast-like cells
Grantee:Fernanda da Silveira Vargas
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/12938-8 - New parameters for tooth bleaching: an evaluation of efficacy, citotoxicity and molecular effects
Grantee:Carlos Alberto de Souza Costa
Support type: Regular Research Grants