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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laser and light-emitting diode effects on pre-osteoblast growth and differentiation

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Pagin, Marina Tochetti [1] ; de Oliveira, Flavia Amadeu [2] ; Oliveira, Rodrigo Cardoso [2] ; Passanezi Sant'Ana, Adriana Campos [3] ; Rubo de Rezende, Maria Lucia [3] ; Aguiar Greghi, Sebastio Luiz [3] ; Damante, Carla Andreotti [3]
Total Authors: 7
[1] Bauru Sch Dent, BR-17012901 Sao Paulo - Brazil
[2] Bauru Sch Dent, Dept Biol Sci, BR-17012901 Sao Paulo - Brazil
[3] Bauru Sch Dent, Discipline Periodont, BR-17012901 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Lasers in Medical Science; v. 29, n. 1, p. 55-59, JAN 2014.
Web of Science Citations: 21

The acceleration of bone regeneration by low-intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. The purpose of this study is to compare the effects of light-emitting diode (LED) and laser on pre-osteoblast MC3T3 proliferation and differentiation. Cells were irradiated with red, infrared, and LED (3 and 5 J/cm(2)). Lasers had a power density of 1 W/cm(2) and irradiation time of 2 and 5 s. LED had a power density of 60 mW/cm(2) and irradiation time of 50 and 83 s. Control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, and 96 h), and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72 and 96 h and 7 and 14 days). At 24 h, the cell growth was enhanced 3.6 times by LED (5 J/cm(2)), 6.8 times by red laser (3 J/cm(2)), and 10.1 times by red laser (5 J/cm(2)) in relation to control group (p < 0.05). At the other periods, there was no influence of irradiation on cell growth (p > 0.05). The production of ALP was not influenced by irradiation at any period of time (p > 0.05). Low-intensity laser and LED have similar effects on stimulation of cell growth, but no effect on cell differentiation. (AU)

FAPESP's process: 10/18361-1 - Comparative evaluation of the effects of low-intesity laser and LED phototherapy on proliferation, metabolism and differentiation of pre-osteoblasts
Grantee:Marina Tochetti Pagin
Support Opportunities: Scholarships in Brazil - Scientific Initiation