Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Methionine concentration in the diet has a tissue-specific effect on chromosomal stability in female mice

Full text
Author(s):
Aissa, Alexandre Ferro [1] ; Ursula Hermogenes Gomes, Tarsila Daysy [2] ; Almeida, Mara Ribeiro [2] ; Hernandes, Livia Cristina [2] ; Castania Darin, Joana D'arc [2] ; Pires Bianchi, Maria Lourdes [2] ; Greggi Antunes, Lusania Maria [1, 2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Food and Chemical Toxicology; v. 62, p. 456-462, DEC 2013.
Web of Science Citations: 8
Abstract

Inadequate nutrient intake can influence the genome. Since methionine is an essential amino acid that may influence DNA integrity due to its role in the one-carbon metabolism pathway, we were interested in whether methionine imbalance can lead to genotoxic events. Adult female Swiss mice were fed a control (0.3% DL-methionine), methionine-supplemented (2.0% DL-methionine) or methionine-deficient (0% DL-methionine) diet over a 10-week period. Chromosomal damage was assessed in peripheral blood using a micronucleus test, and DNA damage was assessed in the liver, heart and peripheral blood tissues using a comet assay. The mRNA expression of the mismatch repair genes Mlh1 and Msh2 was analyzed in the liver. The frequency of micronucleus in peripheral blood was increased by 122% in the methionine-supplemented group (p < 0.05). The methionine-supplemented diet did not induce DNA damage in the heart and liver tissues, but it increased DNA damage in the peripheral blood. The methionine-deficient diet reduced basal DNA damage in liver tissue. This reduction was correlated with decreased mRNA expression of Msh2. Our results demonstrate that methionine has a tissue-specific effect because methionine-supplemented diet induced both chromosomal and DNA damage in peripheral blood while the methionine-deficient diet reduced basal DNA damage in the liver. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 10/01410-0 - Gene-diet interaction in mice supplemented with methionine in the fetal and postnatal periods: studies of genomic instability, methylation and expression of genes related to cardiovascular diseases
Grantee:Alexandre Ferro Aissa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/02365-6 - Methylation profile, genomic instability and gene expression evaluations related with cardiovascular diseases in mice supplemented with methionine diet
Grantee:Lusânia Maria Greggi Antunes
Support type: Regular Research Grants