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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil

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Author(s):
Laurito, Magdalena [1] ; de Oliveira, Tatiane M. P. [2] ; Almiron, Walter Ricardo [1] ; Mureb Sallum, Maria Anice [2]
Total Authors: 4
Affiliation:
[1] Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, Ctr Invest Entomol Cordoba, Inst Invest Biol & Tecnol, RA-5000 Cordoba - Argentina
[2] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01255 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 108, n. 1, p. 110-U152, DEC 2013.
Web of Science Citations: 40
Abstract

Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species (Cx. acharistus, Cx. chidesteri, Cx. dolosus, Cx. lygrus and Cx. saltanensis) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus. Cx. maxi, Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex (Cux.) species. (AU)

FAPESP's process: 11/20397-7 - Morphological and molecular taxonomy and phylogeny of Nyssorhynchus subgenus of Anopheles (Diptera: Culicidae), with special emphasis on Anopheles darlingi from Mata Atlântica
Grantee:Maria Anice Mureb Sallum
Support type: Regular Research Grants