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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients

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Pereira, Weendelly Nayara [1] ; Ferraz, Mariane Avante [1] ; Zabaglia, Luanna Munhoz [1] ; de Labio, Roger William [2] ; Orcini, Wilson Aparecido [1] ; Bianchi Ximenez, Joao Paulo [1] ; Neto, Agostinho Caleman [2] ; Marques Payao, Spencer Luiz [1, 2] ; Rasmussen, Lucas Trevizani [1]
Total Authors: 9
[1] Univ Sagrado Coracao, Bauru, SP - Brazil
[2] Marilia Med Sch, Blood Bank, Dept Genet, Marilia, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 20, JAN 23 2014.
Web of Science Citations: 5

Background: Only a few Helicobacter pylori-infected individuals develop severe gastric diseases and virulence factors of H. pylori appear to be involved in such clinical outcomes. Duodenal ulcer promoting gene A (dupA) is a novel virulence factor of Helicobacter pylori that is associated with duodenal ulcer development and reduced risk for gastric carcinoma in some populations. The aims of the present study were to determine the presence of dupA gene and evaluate the association among dupA and other virulence factors including cagA and vacA in Brazilian patients. Gastric biopsies were obtained from 205 dyspeptic patients (100 children and 105 adults). DNA was extracted and analyzed for the presence of H. pylori and its virulence factors using the polymerase chain reaction method. Results: Patients with gastritis tested positive for H. pylori more frequently. The dupA gene was detected in 41.5% of them (85/205); cagA gene was found in 98 isolates (47.8%) and vacA genotype s1/m1 in 50.2%, s1/m2 in 8.3%, s2/m2 in 36.6%, s2/m1 in 0.5% and s1/s2/m1/m2 in 4.4%. We also verified a significant association between cagA and dupA genes {[} p = 0.0003, relative risk (RR) 1.73 and confidence interval {[} Cl] = 1.3-2.3]. The genotypes s1/m1 were also associated with dupA gene (p = 0.0001, RR: 1.72 and Cl: 1.3-2.2). The same associations were found when analyzing pediatric and adult groups of patients individually. Conclusion: Ours results suggest that dupA is highly frequent in Brazilian patients and is associated with cagA gene and vacA s1/m1 genotype, and it may be considered an important virulence factor in the development of gastric diseases in adults or children. (AU)

FAPESP's process: 12/18333-3 - Characterization of virulence factor dupA, of Helicobacter pylori, genotyping and analysis of the expression of genes of tumor necrosis factor and E-cadherin in samples of children and adults with symptoms peptides
Grantee:Lucas Trevizani Rasmussen
Support type: Regular Research Grants