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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cyclic-di-GMP levels affect Pseudomonas aeruginosa fitness in the presence of imipenem

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Nicastro, Gianlucca G. [1] ; Kaihami, Gilberto H. [1] ; Pereira, Thays O. [1] ; Meireles, Diogo A. [1] ; Groleau, Marie-Christine [2] ; Deziel, Eric [2] ; Baldini, Regina L. [1]
Total Authors: 7
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil
[2] INRS, Inst Armand Frappier, Laval, PQ - Canada
Total Affiliations: 2
Document type: Journal article
Source: ENVIRONMENTAL MICROBIOLOGY; v. 16, n. 5, p. 1321-1333, MAY 2014.
Web of Science Citations: 7

A large number of genes coding for enzymes predicted to synthesize and degrade 3 `-5 `-cyclic diguanylic acid (c-di-GMP) is found in most bacterial genomes and this dinucleotide emerged as an intracellular signal-controlling bacterial behaviour. An association between high levels of c-di-GMP and antibiotic resistance may be expected because c-di-GMP regulates biofilm formation and this mode of growth leads to enhanced antibiotic resistance. However, a clear understanding of this correlation has not been established. We found that increased levels of c-di-GMP in Pseudomonas aeruginosa improve fitness in the presence of imipenem, even when grown as planktonic cells. P. aeruginosa post-transcriptionally regulates the amounts of five porins in response to c-di-GMP, including OprD, responsible for imipenem uptake. Cells with low c-di-GMP levels are consequently more sensitive to this antibiotic. Main efflux pumps or beta-lactamase genes did not show altered mRNA levels in P. aeruginosa strains with modified different c-di-GMP concentrations. Together, our findings show that c-di-GMP levels modulate fitness of planktonic cultures in the presence of imipenem. (AU)

FAPESP's process: 11/08841-9 - Comparative analysis of regulatory mechanisms related to the second messenger c-di-GMP in Pseudomonas and Xanthomonas
Grantee:Regina Lúcia Baldini
Support type: Regular Research Grants
FAPESP's process: 09/09211-9 - Function and regulation of virulence-related genes of the proteobacterium Pseudomonas aeruginosa
Grantee:Regina Lúcia Baldini
Support type: Regular Research Grants
FAPESP's process: 07/03121-2 - Function of Pseudomonas aeruginosa PA14_41070 gene, involved in pathogenicity and quorum sensing
Grantee:Diogo de Abreu Meireles
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 09/08198-9 - Search for targets of the second messenger c-diGMP in Pseudomonas aeruginosa
Grantee:Gianlucca Gonçalves Nicastro
Support type: Scholarships in Brazil - Doctorate