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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of ibuprofen enantiomers in rat plasma by liquid chromatography with tandem mass spectrometry

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Author(s):
Cavalcanti Cardoso, Juciane Lauren [1] ; Lanchote, Vera Lucia [1] ; Marques Pereira, Maria Paula [1] ; de Moraes, Natalia Valadares [2] ; Lepera, Jose Salvador [2]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-05508 Sao Paulo - Brazil
[2] Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Principios Ativos Nat & Toxicol, BR-14801902 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF SEPARATION SCIENCE; v. 37, n. 8, p. 944-949, APR 2014.
Web of Science Citations: 11
Abstract

A sensitive and selective method for the analysis of ibuprofen enantiomers by LC-MS/MS was developed and validated for the purpose of application in pharmacokinetic studies in small experimental animals. Aliquots of 200 L plasma were submitted to liquid-liquid extraction with hexane/diisopropylether (50:50 v/v) in acid pH. Separation was accomplished in a Chirex (R) 3005 (250 x 4.6 mm, 5 m) column at 25 degrees C with a mobile phase that consisted of 0.01 M ammonium acetate in methanol at a flow rate of 1.1 mL/min. The mass spectrometer consisted of an ESI interface operating at negative ionization mode and multiple reaction monitoring. The transitions 205 > 161 and 240 > 197 were monitored for ibuprofen enantiomers and fenoprofen (internal standard), respectively. Method validation included the evaluation of the matrix effect, stability, linearity, lower LOQ, within-run and between-run precision, and accuracy. The lower LOQ was 25 ng/mL for each ibuprofen enantiomer, and the calibration curves showed good linearity in the range 0.025-50 g/mL. The method was successfully applied in the investigation of pharmacokinetic disposition of ibuprofen enantiomers in rats treated orally with 25 mg/kg of the racemate. Enantioselective kinetic disposition was observed with accumulation of (+)-(S)-ibuprofen in rats following single oral administration. (AU)

FAPESP's process: 09/17532-0 - Influence of chronic exposure to automotive fuels in the activity of CYP3A, CYP2C9 and CYP2D in rats treated with chiral drugs
Grantee:Jose Salvador Lepera
Support Opportunities: Regular Research Grants