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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recombinant Leishmania infantum Heat Shock Protein 83 for the Serodiagnosis of Cutaneous, Mucosal, and Visceral Leishmaniases

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Celeste, Beatriz Julieta [1] ; Arroyo Sanchez, Maria Carmen [1] ; Ramos-Sanchez, Eduardo Milton [1] ; Castro, Luiz Guilherme M. [2] ; Lima Costa, Francisco Assis [3] ; Goto, Hiro [1, 4]
Total Authors: 6
[1] Univ Sao Paulo, Inst Med Trop Sao Paulo, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Dermatol, Fac Med, BR-05403000 Sao Paulo - Brazil
[3] Univ Fed Piaui, Dept Clin & Cirurgia Vet, Ctr Ciencias Agr, Teresina, Piaui - Brazil
[4] Univ Sao Paulo, Fac Med, Dept Prevent Med, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: American Journal of Tropical Medicine and Hygiene; v. 90, n. 5, p. 860-865, MAY 2014.
Web of Science Citations: 13

Routine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of Leishmania infantum rHsp83 compared with L. major-like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA-L. major-like when considering either CL/ML (P = 0.041) or all leishmaniasis patients (P = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA-L. major-like, specifically for Chagas disease samples (P < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML (P = 0.607) or CL (P = 0.205). We recommend ELISA-L. infantum-rHsp83 as a routine confirmatory serological assay for the diagnosis of Leishmania infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases. (AU)

FAPESP's process: 11/02235-0 - Efficacy of ELISA-based serological assay with recombinant Leishmania infantum Hsp83 antigen in the diagnosis of leishmaniasis in HIV-infected patients
Grantee:Hiro Goto
Support type: Regular Research Grants