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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of inflammatory response induced by Potamotrygon motoro stingray venom in mice

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Kimura, Louise F. [1] ; Prezotto-Neto, Jose P. [1] ; Antoniazzi, Marta M. [2] ; Jared, Simone G. S. [2] ; Santoro, Marcelo L. [3] ; Barbaro, Katia C. [1]
Total Authors: 6
[1] Inst Butantan, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Cell Biol Lab, BR-05503900 Sao Paulo - Brazil
[3] Inst Butantan, Lab Pathophysiol, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Experimental Biology and Medicine; v. 239, n. 5, p. 601-609, MAY 2014.
Web of Science Citations: 8

Freshwater stingray accidents cause intense pain followed by edema, erythema, and necrosis formation. Treatment for stingray envenomation is based on administration of analgesic, antipyretic, and anti-inflammatory drugs. This report evaluated the local inflammatory reaction-including edema formation, leukocyte recruitment, release of inflammatory mediators, and histopathological changes-after the intraplantar injection of Potamotrygon motoro stingray venom in mice. Edema was observed as soon as 15 min after venom injection, peaking at 30 min, and lasted up to 48 h. In addition, P. motoro venom increased neutrophil counts in the site of injection, at all time periods and venom doses analyzed. Increased eosinophil and lymphocyte counts were detected mainly at 24 h. Moreover, monocytes/macrophages were observed in large amounts at 24 and 48 h. Microscopically, the venom induced leukocyte migration to the injured tissue, edema, mast cell degranulation, angiogenesis, and epidermal damage. Inflammatory mediator release (IL-6, MCP-1 and KC) was detected as soon as 1 h after venom injection, and it increased significantly at 4 h. At 24 h, the venom induced only the production of MCP-1. These results show that this stingray venom evokes a complex inflammatory reaction, with rapid and persistent edema formation, leukocyte recruitment, and release of cytokines and chemokines. (AU)

FAPESP's process: 12/00166-3 - Participation of mast cells and histamine on inflammatory events induced by Potamotrygon motoro stingray venom in murine model
Grantee:Katia Cristina Barbaro Nogueira
Support type: Regular Research Grants