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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rational development of a novel TFPI-like inhibitor from Amblyomma cajennense tick

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Morais, Katia L. P. [1] ; Mesquita Pasqualoto, Kerly Fernanda [1] ; Fernandes Pacheco, Mario Thiego [1] ; Berra, Carolina Maria [1] ; Alvarez-Flores, Miryam Paola [1] ; Chudzinski-Tavassi, Ana Marisa [1]
Total Authors: 6
[1] Inst Butantan, Lab Bioquim & Biofis, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Review article
Source: Toxin Reviews; v. 33, n. 1-2, SI, p. 48-52, MAR-JUN 2014.
Web of Science Citations: 3

Hematophagous organisms have been described to possess specific blood coagulation inhibitors with different structures and novel mechanism of action. A factor Xa inhibitor was identified through the salivary glands transcriptome from the adult Amblyomma cajennense tick. The recombinant form was expressed in Escherichia coli and named Amblyomin-X. The recombinant protein not only inhibits factor X, but is also able to inhibit the activation of factor X by the extrinsic tenase complex. It seems to activate the apoptosis pathway through proteasome inhibition and endoplasmic reticulum-stress. It promotes regression and reduction of lung metastasis in murine melanoma tumor, and does not affect normal cells, emphasizing its selectivity of action. (AU)

FAPESP's process: 11/05969-4 - Investigating the role of dynein in the mechanism of antitumor action of Amblyomin-X in melanoma cells and pancreatic adenocarcinoma
Grantee:Mario Thiego Fernandes Pacheco
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 10/07958-7 - Evaluation of the pro-apoptotic mechanism of action of the Amblyomin-X
Grantee:Kátia Luciano Pereira Morais
Support type: Scholarships in Brazil - Doctorate