| Full text | |
| Author(s): |
Freires, Irlan de Almeida
[1]
;
Murata, Ramiro Mendonca
[2, 3]
;
Furletti, Vivian Fernandes
[4]
;
Sartoratto, Adilson
[4]
;
de Alencar, Severino Matias
[5]
;
Figueira, Glyn Mara
[4]
;
de Oliveira Rodrigues, Janaina Aparecida
[6]
;
Teixeira Duarte, Marta Cristina
[4]
;
Rosalen, Pedro Luiz
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[2] Univ So Calif, Ostrow Sch Dent, Div Periodontol Diagnost Sci & Dent Hyg, Los Angeles, CA - USA
[3] Univ So Calif, Ostrow Sch Dent, Div Biomed Sci, Los Angeles, CA - USA
[4] Univ Estadual Campinas, Res Ctr Chem Biol & Agr, Campinas, SP - Brazil
[5] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agri Food Ind Food & Nutr, Piracicaba, SP - Brazil
[6] Univ Sao Paulo, Sch Engn Sao Carlos, Postgrad Program Interunits Bioengn, Sao Carlos, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | PLoS One; v. 9, n. 6 JUN 5 2014. |
| Web of Science Citations: | 36 |
| Abstract | |
candidiasis is an opportunistic fungal infection of the oral cavity with increasingly worldwide prevalence and incidence Novel specifically-targeted strategies to manage this ailment have been proposed using essential oils CEO) known to have antifungal properties. In this study, we aim to investigate the antifungal activity and mode of action of the EO from Coriandrum sativurn L (coriander) leaves on Candida spp. In addition detected the molecular targets affected in who genorne expression in human cells. The EO phytochernical profile indicates monoterpenes and sesquiterpenes as components, which are likely to negatively impact the viability of yeast cells. There seems to be a synergistic activity of EO chemical compounds as their isolation into fractions led to a decreased antimicrobial effect. C. sativum EO may bind membrane ergosterol, increasing ionic permeability and causing membrane damage leading to cell death, but it does not on cell wall biosynthesis-related pathways. This mode of action is illustrated by photomicrographs showing disruption in caused by the EO at varied concentrations. The EO also inhibited Candida biofilm adherence to polystyrene substrate at low concentrations, and decreased the proteolytic activity of Candida albicans at mini inhibitory concentration. Finally, the EO and its selected active fraction had low cytotoxicity on human cells, mechanisms affecting gene expression in pathways involving chernokines and MAP-kinase (proliferation/apoptosis), as adhesion proteins. These findings highlight the potential antifungal activity of the EO from C. sativum leaves and suggest avenues for future translational toxicological research. (AU) | |
| FAPESP's process: | 11/14757-0 - Evaluation of antimicrobial activity of essential oils on oral pathogens |
| Grantee: | Pedro Luiz Rosalen |
| Support type: | Regular Research Grants |
| FAPESP's process: | 11/15984-0 - Anti-Candida, antiproliferative and cytotoxic activities evaluation of essential oil and fractions from Coriandrum sativum L. |
| Grantee: | Irlan de Almeida Freires |
| Support type: | Scholarships in Brazil - Master |