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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nitrogen-15 labeling of Crotalaria juncea green manure

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Edmilson José Ambrosano ; Paulo Cesar Ocheuze Trivelin ; Heitor Cantarella ; Raffaella Rossetto ; Takashi Muraoka ; José Albertino Bendassolli ; Gláucia Maria Bovi Ambrosano ; Luciano Grassi Tamiso ; Felipe de Campos Vieira ; Ithamar Prada Neto
Total Authors: 10
Document type: Journal article
Source: Scientia Agricola; v. 60, n. 1, p. 181-184, Fev. 2003.

Most studies dealing with the utilization of 15N labeled plant material do not present details about the labeling technique. This is especially relevant for legume species since biological nitrogen fixation difficults plant enrichment. A technique was developed for labeling leguminous plant tissue with 15N to obtain labeled material for nitrogen dynamics studies. Sun hemp (Crotalaria juncea L.) was grown on a Paleudalf, under field conditions. An amount of 58.32 g of urea with 70.57 ± 0.04 atom % 15N was sprayed three times on plants grown on eight 6-m²-plots. The labelled material presented 2.412 atom % 15N in a total dry matter equivalent to 9 Mg ha-1 This degree of enrichment enables the use of the green manure in pot or field experiments requiring 15N-labeled material. (AU)