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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The in vitro maintenance of clock genes expression within the rat pineal gland under standard and norepinephrine-synchronized stimulation

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Author(s):
Andrade-Silva, Jessica [1] ; Cipolla-Neto, Jose [1] ; Peliciari-Garcia, Rodrigo A. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: NEUROSCIENCE RESEARCH; v. 81-82, p. 1-10, APR-MAY 2014.
Web of Science Citations: 5
Abstract

Although the norepinephrine (NE) synchronization protocol was proved to be an important procedure for further modulating in vitro pineal melatonin synthesis, the maintenance of clock genes under the same conditions remained to be investigated. The aim of this study was to investigate the maintenance of the clock genes expression in pineal gland cultures under standard and NE-synchronized stimulation. The glands were separated into three experimental groups: Control, Standard (acute NE-stimulation), and NE-synchronized. The expression of Bmal1, Per2, Cry2, Rev-erb alpha, the clock controlled gene Dbp and Arylalkylamine-N-acetyltransferase were investigated, as well as melatonin content. No oscillations were observed in the expression of the investigated genes from the control group. Under Standard NE stimulation, the clock genes did not exhibit a rhythmic pattern of expression. However, in the NE-synchronized condition, a rhythmic expression pattern was observed in all cases. An enhancement in pineal gland responsiveness to NE stimulation, reflected in an advanced synthesis of melatonin was also observed. Our results reinforce our previous hypothesis that NE synchronization of pineal gland culture mimics the natural rhythmic release of NE in the gland, increasing melatonin synthesis and keeping the pineal circadian clock synchronized, ensuring the fine adjustments that are relied in the clockwork machinery. (C) 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. (AU)

FAPESP's process: 07/07790-6 - Role of insulin on the regulation of melatonin synthesis by pineal gland: action mechanisms and functional characteristics
Grantee:Rodrigo Antonio Peliciari Garcia
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 09/52920-0 - Melatonin and the control of energy metabolism: central and peripheral actions and the circadian timing of the metabolic function
Grantee:José Cipolla Neto
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/04359-8 - Role of noradrenergic synchronized on melatonin synthesis of pineal gland culture: functionals characteristics and action mechanisms
Grantee:Jéssica Andrade da Silva
Support type: Scholarships in Brazil - Master