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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bone marrow aspirate combined with low-level laser therapy: A new therapeutic approach to enhance bone healing

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Author(s):
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Nagata, Maria J. H. [1] ; Santinoni, Carolina S. [2] ; Pola, Natalia M. [3] ; de Campos, Natalia [4] ; Messora, Michel R. [5] ; Bomfim, Suely R. M. [6] ; Ervolino, Edilson [7] ; Fucini, Stephen E. ; Faleiros, Paula L. [9] ; Garcia, Valdir G. [10] ; Bosco, Alvaro F. [11]
Total Authors: 11
Affiliation:
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[1] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
[2] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
[3] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
[4] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
[5] Univ Sao Paulo. Sch Dent Ribeirao Preto
[6] Univ Estadual Paulista UNESP. Vet Sch Aracatuba
[7] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
[9] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
[10] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
[11] Univ Estadual Paulista UNESP. Dent Sch Aracatuba
Total Affiliations: 11
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 121, p. 6-14, APR 5 2013.
Web of Science Citations: 4
Abstract

This study evaluated the influence of bone marrow aspirate (BMA), low-level laser therapy (LLLT) and their combination on bone healing in surgically created critical-size defects (CSDs) in rat calvaria. 40 rats were divided into four groups: C (control), BMA, LLLT and BMA/LLLT. A 5 mm diameter CSD was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group BMA, the defect was filled with BMA. In groups LLLT and BMA/LLLT, the defect received laser irradiation (InGaAlP laser), was filled with blood clot or BMA respectively, and irradiated again. Animals were euthanized 30 days postoperatively. Histomorphometric and immunohistochemical analyses were performed. Newly formed bone area (NFBA) was calculated as percentage of the total area of the original defect. Proliferating cell nuclear antigen (PCNA), runt-related transcription factor 2 (Runx2) and osteocalcin (OCN) immunohistochemical staining were performed. PCNA-positive, Runx2-positive and OCN-positive cells were quantified. Data were statistically analyzed. Group BMA/LLLT had significantly greater NFBA than groups C, BMA or LLLT. Group BMA presented significantly greater NFBA than control, while group LLLT did not. Group BMA/LLLT presented a significantly higher number of PCNA-positive and OCN-positive cells than any of the other groups. Groups BMA/LLLT and BMA showed a significantly lower number of Runx2-positive cells than groups C or LLLT. The combination of BMA/LLLT yielded significantly greater bone formation in surgically created CSD in rat calvaria when compared to control, or either treatment alone. (C) 2013 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/04450-2 - Bone healing in crtical-size defects treated with either Bone Marrow Aspirate, Low-Level Laser Therapy or a combination of both therapies: a histologic and histometric study in rat calvaria.
Grantee:Carolina dos Santos Santinoni
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/18925-2 - Bone healing in critical-size defects treated with bone marrow aspirate. Histological, histometric and immunoistochemical study in rat calvarial
Grantee:Maria José Hitomi Nagata
Support type: Regular Research Grants