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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi

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Araujo, Adriano Fernando [1, 2] ; de Oliveira, Gabriel [3] ; Vasconcelos, Juliana Fraga [4, 5] ; Ersching, Jonatan [1, 2] ; Dominguez, Mariana Ribeiro [1, 2] ; Vasconcelos, Jose Ronnie [1, 2, 6] ; Machado, Alexandre Vieira [7] ; Gazzinelli, Ricardo Tostes [7, 8, 9] ; Bruna-Romero, Oscar [10] ; Soares, Milena Botelho [4, 5] ; Rodrigues, Mauricio Martins [1, 2]
Total Authors: 11
[1] Univ Fed Sao Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, BR-04044010 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044010 Sao Paulo - Brazil
[3] Fiocruz MS, Inst Oswaldo Cruz, Lab Biol Celular, BR-21040360 Rio De Janeiro, RJ - Brazil
[4] Fiocruz MS, Ctr Pesquisas Goncalo Moniz, BR-40296710 Salvador, BA - Brazil
[5] Hosp Sao Rafael, BR-41253190 Salvador, BA - Brazil
[6] UNIFESP, Inst Saude Soc, Dept Biociencias, BR-11015020 Santos, SP - Brazil
[7] Fiocruz MS, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG - Brazil
[8] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG - Brazil
[9] Univ Massachusetts, Sch Med, Dept Med, Div Infect Dis & Immunol, Worcester, MA 01655 - USA
[10] Univ Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, BR-88040900 Florianopolis, SC - Brazil
Total Affiliations: 10
Document type: Journal article
Source: Mediators of Inflammation; 2014.
Web of Science Citations: 0

In earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replication-defective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite. Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged with myotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing the murine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptible male mice against acute lethal infections with these parasites. The prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T. cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease. (AU)

FAPESP's process: 09/06820-4 - Characterization of antigen presenting cells capable of initiating the immune response and control the immunodominance during Trypanosoma cruzi infection
Grantee:Maurício Martins Rodrigues
Support type: Regular Research Grants
FAPESP's process: 12/22514-3 - Migration study of specific T cells generated by vaccination or Trypanosoma cruzi infection
Grantee:Jose Ronnie Carvalho de Vasconcelos
Support type: Research Grants - Young Investigators Grants