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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inhibition of peripheral anion exchanger 3 decreases formalin-induced pain

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Author(s):
Barragan-Iglesias, Paulino [1] ; Rocha-Gonzalez, Hector I. [2] ; Baruch Pineda-Farias, Jorge [1] ; Murbartian, Janet [1] ; Godinez-Chaparro, Beatriz [3] ; Reinach, Peter S. [4] ; Cunha, Thiago M. [4] ; Cunha, Fernando Q. [4] ; Granados-Soto, Vinicio [1]
Total Authors: 9
Affiliation:
[1] CINVESTAV, Dept Farmacobiol, Neurobiol Pain Lab, Ctr Invest & Estudios Avanzados, Mexico City 14330, DF - Mexico
[2] Inst Politecn Nacl, Escuela Super Med, Secc Estudios Posgrad & Invest, Mexico City, DF - Mexico
[3] Univ Autonoma Metropolitana Xochimilco, Dept Sistemas Biol, Div Ciencias Biol & Salud, Mexico City, DF - Mexico
[4] Univ Sao Paulo, Riberao Preto Med Sch, Dept Pharmacol, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: European Journal of Pharmacology; v. 738, p. 91-100, SEP 5 2014.
Web of Science Citations: 4
Abstract

We determined the role of chloride-bicarbonate anion exchanger 3 in formalin-induced acute and chronic rat nociception. Formalin (1%) produced acute (first phase) and tonic (second phase) nociceptive behaviors (flinching and licking/lifting) followed by long-lasting evoked secondary mechanical allodynia and hyperalgesia in both paws. Local peripheral pre-treatment with the chloride-bicarbonate anion exchanger inhibitors 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid and 4-acetamido-4'-isothiocyanato-2,2'-stilbenedisulfonic acid prevented formalin-induced nociception mainly during phase 2. These drugs also prevented in a dose-dependent fashion long-lasting evoked secondary mechanical allodynia and hyperalgesia in both paws. Furthermore, post-treatment (on day 1 or 6) with 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid reversed established hypersensitivity. Anion exchanger 3 was expressed in dorsal root ganglion neurons and it co-localized with neuronal nuclei protein (NeuN), substance P and purinergic P2X3 receptors. Furthermore, Western blot analysis revealed a band of about 85 kDa indicative of anion exchanger 3 protein expression in dorsal root ganglia of naive rats, which was enhanced at 1 and 6 days after 1% formalin injection. On the other hand, this rise failed to occur during 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid exposure. These results suggest that anion exchanger 3 is present in dorsal root ganglia and participates in the development and maintenance of short and long-lasting formalin-induced nociception. (C) 2014 Elsevier By. All rights reserved. (AU)

FAPESP's process: 11/19670-0 - Mechanisms involved in the pathophysiology of rheumatoid arthritis, pain and sepsis
Grantee:Fernando de Queiroz Cunha
Support type: Research Projects - Thematic Grants