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Entree

Structural genomics of cyclin dependent kinases and plant defensive proteinases and their natural inhibitors

Processo: 01/07532-0
Linha de fomento:Auxílio à Pesquisa - Regular
Vigência: 01 de outubro de 2001 - 30 de setembro de 2005
Área do conhecimento:Ciências Biológicas - Biofísica
Pesquisador responsável:Walter Filgueira de Azevedo Junior
Beneficiário:Walter Filgueira de Azevedo Junior
Instituição-sede: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brasil
Bolsa(s) vinculada(s):02/10294-7 - Structural genomics of cyclin dependent kinases and plant defensive proteinases and their natural inhibitors, BP.TT
Assunto(s):Peptídeo hidrolases 

Resumo

Considering the scientific relevance and the previous experience of the researchers involved in the project we will focus our structural genomics project on two fronts: CDKs and sugar cane proteins. 1) CDKs. Cell cycle progression is tightly controlled by the activity of CDKs. Since deregulation of cyclins and/or alteration or absence of CDK inhibitors (CKIs) have been associated with many cancers, there is strong interest in structural studies of CDKs (Azevedo et al. 1996, 1997). The present project will focus on three CDKs, CDK8, CDK9 and CDK10. 2) Sugar-cane proteins. The potential for using host plant resistance as a tool in pest control has increased with the development of gene transfer techniques. The synthesis of antimetabolic proteins, like Pls, which interfere with the digestive process in insects, is a defense strategy that plants use extensively. Furthermore, the existence of natural proteinases inhibitors in plants is very important for the regulatory activity of these enzymes in plant development and resistance against diseases and infections. (AU)

Publicações científicas (22)
(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)
CANDURI‚ F.; CARDOSO PEREZ‚ P.; CACERES‚ R.A.; DE AZEVEDO‚ W.F. Protein kinases as targets for antiparasitic chemotherapy drugs. CURRENT DRUG TARGETS, v. 8, n. 3, p. 389-398, 2007.
DELATORRE‚ P.; ROCHA‚ B.; SOUZA‚ E.; OLIVEIRA‚ T.; BEZERRA‚ G.; MORENO‚ F.; FREITAS‚ B.; SANTI-GADELHA‚ T.; SAMPAIO‚ A.; AZEVEDO‚ W.; OTHERS. Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules. BMC STRUCTURAL BIOLOGY, v. 7, n. 1, p. 52, 2007.
DIAS‚ B.; MARCIO‚ V.; ELY‚ F.; PALMA‚ M.S.; DE AZEVEDO‚ W.F.; BASSO‚ L.A.; SANTOS‚ D.S. Chorismate synthase: an attractive target for drug development against orphan diseases. CURRENT DRUG TARGETS, v. 8, n. 3, p. 437-444, 2007.
JOSE FREITAS DA SILVEIRA‚ N.; EDUARDO BONALUMI‚ C.; ANDRADE ARCURI‚ H.; FILGUEIRA DE AZEVEDO JUNIOR‚ W. Molecular Modeling Databases: A New Way in the Search of Protein Targets for Drug Development. Current Bioinformatics, v. 2, n. 1, p. 1-10, 2007.
PEREIRA‚ J.H.; VASCONCELOS‚ I.B.; OLIVEIRA‚ J.S.; CACERES‚ R.A.; DE AZEVEDO‚ W.F.; BASSO‚ L.A.; SANTOS‚ D.S. Shikimate kinase: a potential target for development of novel antitubercular agents. CURRENT DRUG TARGETS, v. 8, n. 3, p. 459-468, 2007.
RIBEIRO MARQUES‚ M.; HENRIQUE PEREIRA‚ J.; OLIVEIRA‚ J.S.; AUGUSTO BASSO‚ L.; FILGUEIRA DE AZEVEDO‚ W.; SANTIAGO SANTOS‚ D.; SERGIO PALMA‚ M. The inhibition of 5-enolpyruvylshikimate-3-phosphate synthase as a model for development of novel antimicrobials. CURRENT DRUG TARGETS, v. 8, n. 3, p. 445-457, 2007.
LEOPOLDINO, ANDREIA MACHADO; CANDURI, FERNANDA; CABRAL, HAMILTON; JUNQUEIRA, MAGNO; MARQUI, ALESSANDRA BERNADETE TROVÓ DE; APPONI, LUCIANO H.; FONSECA, ISABEL OSÓRIO DA; DOMONT, GILBERTO BARBOSA; SANTOS, DIÓGENES S.; VALENTINI, SANDRO; ET AL. Expression, purification, and circular dichroism analysis of human CDK9. Protein Expression and Purification, v. 47, n. 2, p. 614-620, June 2006.
SILVEIRA, NELSON JOSÉ FREITAS DA; UCHÔA, HUGO BRANDÃO; PEREIRA, JOSÉ HENRIQUE; CANDURI, FERNANDA; BASSO, LUIZ AUGUSTO; PALMA, MÁRIO SÉRGIO; SANTOS, DIÓGENES SANTIAGO; AZEVEDO JR., WALTER FILGUEIRA DE. Molecular models of protein targets from Mycobacterium tuberculosis. Journal of Molecular Modeling, v. 11, n. 2, p. 160-166, Mar. 2005.
AZEVEDO JR, WALTER FILGUEIRA DE; CANDURI, FERNANDA; FADEL, VALMIR; BASSO, LUIZ AUGUSTO; PALMA, MÁRIO SÉRGIO; SANTOS, DIÓGENES SANTIAGO. New catalytic mechanism for human purine nucleoside phosphorylase. Biochemical and Biophysical Research Communications, v. 327, n. 3, p. 646-649, Feb. 2005.
CANDURI, FERNANDA; FADEL, VALMIR; DIAS, MARCIO VINÍCIUS BERTACINE; BASSO, LUIZ AUGUSTO; PALMA, MÁRIO SÉRGIO; SANTOS, DIÓGENES SANTIAGO; AZEVEDO JÚNIOR, WALTER FILGUEIRA DE. Crystal structure of human PNP complexed with hypoxanthine and sulfate ion. Biochemical and Biophysical Research Communications, v. 326, n. 2, p. 335-338, Jan. 2005.
CANDURI, FERNANDA; DE AZEVEDO, JR., WALTER FILGUEIRA. Structural Basis for Interaction of Inhibitors with Cyclin-Dependent Kinase 2. CURRENT COMPUTER-AIDED DRUG DESIGN, v. 1, n. 1, p. 53-64, JAN 2005. Citações Web of Science: 26.
DA SILVEIRA‚ N.J.F.; ARCURI‚ H.A.; BONALUMI‚ C.E.; DE SOUZA‚ F.P.; MELLO‚ I.M.; RAHAL‚ P.; PINHO‚ J.R.R.; DE AZEVEDO‚ W.F. Molecular models of NS3 protease variants of the Hepatitis C virus. BMC STRUCTURAL BIOLOGY, v. 5, n. 1, p. 1, 2005.
FADEL‚ V.; BETTENDORFF‚ P.; HERRMANN‚ T.; DE AZEVEDO JR‚ W.F.; OLIVEIRA‚ E.B.; YAMANE‚ T.; WÜTHRICH‚ K. Automated NMR structure determination and disulfide bond identification of the myotoxin crotamine from Crotalus durissus terrificus. Toxicon, v. 46, n. 7, p. 759-767, 2005.
PEREIRA, JOSÉ HENRIQUE; OLIVEIRA, JAIM SIMÕES DE; CANDURI, FERNANDA; DIAS, MARCIO VINICIUS BERTACINE; PALMA, MÁRIO SÉRGIO; BASSO, LUIZ AUGUSTO; SANTOS, DIÓGENES SANTIAGO; AZEVEDO JUNIOR, WALTER FILGUEIRA DE. Structure of shikimate kinase from Mycobacterium tuberculosis reveals the binding of shikimic acid. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v. 60, n. 2, p. 2310-2319, Dec. 2004.
PEREIRA, JOSÉ HENRIQUE; OLIVEIRA, JAIM SIMÕES DE; CANDURI, FERNANDA; DIAS, MARCIO VINICIUS BERTACINE; PALMA, MÁRIO SÉRGIO; BASSO, LUIZ AUGUSTO; AZEVEDO JÚNIOR, WALTER FILGUEIRA DE; SANTOS, DIÓGENES SANTIAGO. Interaction of shikimic acid with shikimate kinase. Biochemical and Biophysical Research Communications, v. 325, n. 1, p. 10-17, Dec. 2004.
PERES, P.; LOMBARDI, F. R.; SANTOS, G. C. DOS; OLIVIERI, J. R.; CANDURI, F.; BONILLA-RODRIGUEZ, G. O.; AZEVEDO JÚNIOR, W. F. DE. Molecular modeling and small angle X-ray scattering studies of Hoplosternum littorale cathodic haemoglobin. Biochemical and Biophysical Research Communications, v. 325, n. 2, p. 487-493, Dec. 2004.
RIBEIRO, SUSAN PEREIRA; MENDES, MARIA ANITA; SANTOS, LUCILENE DELAZARI DOS; SOUZA, BIBIANA MONSON DE; MARQUES, MAURÍCIO RIBEIRO; AZEVEDO JUNIOR, WALTER FILGUEIRA DE; PALMA, MARIO SERGIO. Structural and functional characterization of N-terminally blocked peptides isolated from the venom of the social wasp Polybia paulista. Peptides, v. 25, n. 12, p. 2069-2078, Dec. 2004.
UCHÔA, HUGO BRANDÃO; JORGE, GUILHERME EBERHART; SILVEIRA, NELSON JOSÉ FREITAS DA; CAMERA JÚNIOR, JOÃO CARLOS; CANDURI, FERNANDA; AZEVEDO JÚNIOR, WALTER FILGUEIRA DE. Parmodel: a web server for automated comparative modeling of proteins. Biochemical and Biophysical Research Communications, v. 325, n. 4, p. 1481-1486, Dec. 2004.
CANDURI, FERNANDA; UCHÔA, HUGO BRANDÃO; AZEVEDO JÚNIOR, WALTER FILGUEIRA DE. Molecular models of cyclin-dependent kinase 1 complexed with inhibitors. Biochemical and Biophysical Research Communications, v. 324, n. 2, p. 661-666, Nov. 2004.
DIAS, MARCIO VINICIUS BERTACINE; ELY, FERNANDA; CANDURI, FERNANDA; PEREIRA, JOSÉ HENRIQUE; FRAZZON, JEVERSON; BASSO, LUIZ AUGUSTO; PALMA, MÁRIO SÉRGIO; AZEVEDO JUNIOR, WALTER FILGUEIRA DE; SANTOS, DIÓGENES SANTIAGO. Crystallization and preliminary X-ray crystallographic analysis of chorismate synthase from Mycobacterium tuberculosis. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v. 60, n. 11, p. 2003-2005, Nov. 2004.
SILVEIRA, NELSON JOSÉ FREITAS DA; UCHÔA, HUGO BRANDÃO; CANDURI, FERNANDA; PEREIRA, JOSÉ HENRIQUE; CAMERA JÚNIOR, JOÃO CARLOS; BASSO, LUIZ AUGUSTO; PALMA, MÁRIO SERGIO; SANTOS, DIÓGENES SANTIAGO; AZEVEDO JÚNIOR, WALTER FILGUEIRA DE. Structural bioinformatics study of PNP from Schistosoma mansoni. Biochemical and Biophysical Research Communications, v. 322, n. 1, p. 100-104, Sept. 2004.
AZEVEDO JUNIOR, WALTER FILGUEIRA DE; SANTOS, GIOVANNI CÉSAR DOS; SANTOS, DENIS MARANGONI DOS; OLIVIERI, JOHNNY RIZZIERI; CANDURI, FERNANDA; SILVA, RAFAEL GUIMARÃES; BASSO, LUIZ AUGUSTO; RENARD, GABY; FONSECA, ISABEL OSÓRIO DA; MENDES, MARIA ANITA; ET AL. Docking and small angle X-ray scattering studies of purine nucleoside phosphorylase. Biochemical and Biophysical Research Communications, v. 309, n. 4, p. 923-928, Oct. 2003.

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