Biodiversidade vegetal e de organismos edáficos em ecossistemas de Araucaria angus...
- Auxílios pontuais (curta duração)
Processo: | 08/11003-2 |
Linha de fomento: | Auxílio à Pesquisa - Publicações científicas - Artigo |
Vigência: | 01 de janeiro de 2009 - 30 de junho de 2009 |
Área do conhecimento: | Ciências Agrárias - Agronomia - Ciência do Solo |
Pesquisador responsável: | Elke Jurandy Bran Nogueira Cardoso |
Beneficiário: | Elke Jurandy Bran Nogueira Cardoso |
Instituição-sede: | Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brasil |
Assunto(s): | Araucaria angustifolia Mycorrhizae DNA espaçador ribossômico |
Resumo
The Fungal Ribosomal Intergenic Spacer Analysis (F-RISA) was used to characterize soil fungal communities from threeecosystems of Araucaria angustifolia from Brazil: a native forest and two replanted forest ecosystems, one of them with apast history of wildfire. The arbuscular mycorrhizal fungi (AMF) infection was evaluated in Araucaria roots of 18-montholdaxenic plants previously inoculated with soils collected from those areas in a greenhouse experiment. The principalcomponent analysis of F-RISA profiles showed different soil fungal community between the three studied areas. Sixty threepercent of F-RISA fragments amplified in the soil and the substrate samples presented lengths between 500 and 700 bp. Thenumber of Operational Taxonomic Units (OTUs) was 34 for soil and 38 for substrate, however, more fragments weredetected in soil (214) than in substrate (163). An in silico F-RISA analysis to compare our data with ITS1-5.8S-ITS2sequences from NCBI database showed the presence of Ascomycota, Basidiomycota and Glomeromycota among the soiland substrate fungal communities. AMF infection was higher in plants inoculated with soil from the native forest and thereplanted forest with wildfire, both presenting similar chemical characteristics but with different disturbance levels. Theseresults indicate that soil chemical composition may influence the soil fungal community structures rather than theanthropogenic or fire disturbances. (AU)