Avaliação dos riscos ambientais da tintura de rede 1 dispersos em rios brasileiros

• Processo: 13/50028-9
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de abril de 2013
• Data de Término da vigência: 31 de março de 2015
• Área do conhecimento: Ciências Exatas e da Terra - Química - Química Analítica
• Acordo de Cooperação: CNRS
• Pesquisador responsável: Maria Valnice Boldrin
• Beneficiário: Maria Valnice Boldrin
• Pesquisador Responsável no exterior: Alain Devaux
• Instituição Parceira no exterior: Université Claude Bernard Lyon 1, França
• Instituição Sede: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brasil
• Vinculado ao auxílio: 08/10449-7 - Avaliação da ocorrência, toxicidade/genotoxicidade e processos para degradação de corantes em efluentes e águas superficiais, AP.TEM
• Assunto(s): Riscos ambientais Rios Corantes Genotoxicidade Salmonella Cromatografia líquida de alta eficiência
• Palavra(s)-Chave do Pesquisador: Disperse Red 1 | Dye Contamination | Genotoxicity | Hplc Ms Ms Qtrap | Photoactivation | Salmonella Assay

Resumo

Dyes are used in the coloration of different substrates, including paper, leather and plastics, but the most important use is on textiles. Up to 15% of the dyes used for coloring textiles might be lost into the environment. Azo dyes can be mutagenic and carcinogenic depending on their chemical structure. They can be found in river's water and sediment and in effluent discharges in the range of μ/L and in the sediment in μ/g. Also several papers reported that effluents from textile mills exhibit mutagenic activity and Japanese scientists discovered a new class of compounds, the phenylbenzotriazoles (PBTAs) derived from dyes, but not coloured that were accounting for at least 50% of the mutagenicity of Japanese rivers. Azo dyes can be toxic to aquatic organisms but although these compounds as well as their transformation products have been found in aquatic ecosystems, no mutagenicity data are available until now in aquatic organisms. Many studies have demonstrated that DNA damage measurement represents a very sensitive biomarker of exposure in aquatic species that can be studied both in vivo and in vitro using fish cell lines and recent studies have clearly demonstrated that genotoxic events in aquatic species could lead to reproduction impairment, subsequently affecting population dynamics. A recent study showed that the commercial dye Disperse Red 1 was preliminary detected in a Brazilian wastewater treatment plant effluent and this dye exhibited high toxicity especially to cladocerans and algae. The NOEC was 0,1 mg/L for both species. This commercial dye also presented mutagenic ativity in the Salmonella microsome assay. Therefore the objectives of this work were: monitor Disperse red 1 dye in Sao Paulo State aquatic ecosystems using Thin Layer Chromatograpy and HPLC MS/MS QTrap, assess its genotoxicity using both in vitro (fish cell lines) and in vivo in fish exposed to dye effluents in the field and verify the effect of photoactivation on the mutagenicity of Disperse Red 1 dye. (AU)