Modificações pós-traducionais para o diagnóstico de câncer e doenças parasitárias: abordagens metodológicas e implicações biológicas

• Processo: 14/06863-3
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Vigência: 01 de fevereiro de 2015 - 30 de setembro de 2019
• Área do conhecimento: Ciências Biológicas - Bioquímica - Química de Macromoléculas
• Pesquisador responsável: Giuseppe Palmisano
• Beneficiário: Giuseppe Palmisano
• Instituição Sede: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Pesquisadores associados: Cláudio Romero Farias Marinho ; Fernando Augusto Soares ; Vilma Regina Martins
• Auxílios(s) vinculado(s): 18/15549-1 - Modificações pós-traducionais nos processos biológicos e no diagnóstico da Doença de Chagas: novas abordagens metodológicas e implicações biológicas, AP.JP2; 18/18257-1 - EMU concedido no processo 14/06863-3: sistema de cromatografia líquida configurado para análise de carboidratos, aminoácidos, peptídeos e glicoproteínas, AP.EMU; 16/50356-4 - MYC: a regulator of exosome protein cargo?, AP.R SPRINT
• Bolsa(s) vinculada(s): 19/04744-0 - Treinamento em técnicas de espectrometria de massas aplicada a estudos proteômicos e análise de modificações pós-traducionais, BE.PC; 19/01798-2 - Implementação e otimização de plataformas computacionais para o aprimoramento das ferramentas de análise e integração de dados de OMICs, BP.TT; 18/13283-4 - Descoberta de potências biomarcadores da Doença de Chagas em urina utilizando técnicas de espectrometria de massas, BP.DR; + mais bolsas vinculadas 18/12349-1 - Modificações pós-traducionais para o diagnóstico de câncer e doenças parasitárias: abordagens metodológicas e implicações biológicas, BP.TT; 17/22060-6 - Modificações pós-traducionais para o diagnóstico de câncer e doenças parasitárias: abordagens metodológicas e implicações biológicas, BP.TT; 16/23689-2 - Identificação da variabilidade proteômica e suas modificações nas Leishmanias L. (V.) braziliensis, L. (L.) amazonensis e L. (L.) chagasi, utilizando uma abordagem shotgun protemics., BP.MS; 16/16071-2 - Modificações pós-traducionais para o diagnóstico de câncer e doenças parasitárias: abordagens metodológicas e implicações biológicas, BP.TT; 15/02866-0 - Descoberta e verificação de glicopeptídeos intactos como candidatos à biomarcadores em câncer de próstata utilizando abordagens de proteômica-baseada em espectrometria de massas, BP.PD - menos bolsas vinculadas
• Assunto(s): Diagnóstico clínico Neoplasias Doenças transmissíveis Proteômica Espectroscopia de massa
• Palavra(s)-Chave do Pesquisador: câncer | Infectious diseases | mass spectrometry | Post-translational modifications | Proteomics | Mass spectrometry applied to proteins

Resumo

Um programa de pesquisa inovador será estabelecido com o objetivo de estudar a modulação das modificações pós-traducionais de proteínas (PTMs), tais como fosforilação, glicosilação e oxidação, em condições patológicas, como a metástase do câncer e para o diagnóstico de infecções parasitárias. O projeto será desenvolvido em três linhas de pesquisa: 1) otimização e desenvolvimento de métodos de enriquecimento/análise usando espectrometria de massas para estudar modificações pós-traducionais em diferentes sistemas biológicos; 2) Desenvolvimento de uma estratégia para a extração e análise de proteínas e peptídeos modificados originados do fluido intersticial de câncer de próstata e de tecidos saudáveis; 3) Desenvolvimento de um método de espectrometria de massas para a detecção em fluidos biológicos de biomarcadores de doenças parasitárias com a intenção de estabelecer um método diagnóstico fácil, sensível e robusto capaz de detectar as doenças em seus estágios iniciais. (AU)

Publicações científicas

(51)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

BOYSEN, ANDERS; PALMISANO, GIUSEPPE; KROGH, THOGER JENSEN; DUGGIN, IAIN G.; LARSEN, MARTIN R.; MOLLER-JENSEN, JAKOB. A novel mass spectrometric strategy ``BEMAP{''} reveals Extensive O-linked protein glycosylation in Enterotoxigenic Escherichia coli. SCIENTIFIC REPORTS, v. 6, AUG 26 2016. (14/06863-3)

MANSO ALVES, MARIA JULIA; KAWAHARA, REBECA; VINER, ROSA; COLLI, WALTER; MATTOS, ELICIANE CEVOLANI; THAYSEN-ANDERSEN, MORTEN; LARSEN, MARTIN ROSSEL; PALMISANO, GIUSEPPE. Comprehensive glycoprofiling of the epimastigote and trypomastigote stages of Trypanosoma cruzi. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 182-192, JAN 16 2017. (14/25494-9, 13/16478-7, 14/06863-3)

CHATTERJEE, SAYANTANI; LEE, LING Y.; KAWAHARA, REBECA; ABRAHAMS, JODIE L.; ADAMCZYK, BARBARA; ANUGRAHAM, MERRINA; ASHWOOD, CHRISTOPHER; SUMER-BAYRAKTAR, ZEYNEP; BRIGGS, MATTHEW T.; CHIK, JENNY H. L.; et al. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. PROTEOMICS, v. 19, n. 21-22, SI, NOV 2019. (17/03010-8, 18/15549-1, 15/02866-0, 14/06863-3)

PIMENTEL, ANDRE C.; FUZITA, FELIPE J.; PALMISANO, GIUSEPPE; FERREIRA, CELIA; TERRA, WALTER R.. Role of cathepsins D in the midgut of Dysdercus peruvianus. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 204, p. 45-52, FEB 2017. (14/06863-3)

MARTINS, LARISSA ALMEIDA; PALMISANO, GIUSEPPE; CORTEZ, MAURO; KAWAHARA, REBECA; DE FREITAS BALANCO, JOSE MARIO; FUJITA, ANDRE; ALONSO, BEATRIZ IGLESIAS; BARROS-BATTESTI, DARCI MORAES; BRAZ, GLORIA REGINA CARDOSO; TIRLONI, LUCAS; et al. The intracellular bacterium Rickettsia rickettsii exerts an inhibitory effect on the apoptosis of tick cells. PARASITES & VECTORS, v. 13, n. 1, DEC 1 2020. (12/24105-3, 18/15549-1, 18/18257-1, 14/11513-1, 07/57749-2, 14/06863-3, 14/05855-7, 13/26450-2)

CAPELLI-PEIXOTO, JANAINA; MULE, SIMON NGAO; TANO, FABIA TOMIE; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteomics and Leishmaniasis: Potential Clinical Applications. PROTEOMICS CLINICAL APPLICATIONS, v. 13, n. 6, AUG 2019. (17/26197-6, 17/04287-3, 14/26777-4, 14/06863-3, 18/18257-1, 18/15549-1, 17/04032-5)

DE OLIVEIRA, GILBERTO SANTOS; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; AVILA, CARLA CRISTI; TEIXEIRA, MARTA M. G.; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Development of a Trypanosoma cruzi strain typing assay using MS2 peptide spectral libraries (Tc-STAMS2). PLoS Neglected Tropical Diseases, v. 12, n. 4, APR 2018. (14/06863-3, 15/02866-0)

AVILA, C. C.; ALMEIDA, F. G.; PALMISANO, G.. Direct identification of trypanosomatids by matrix-assisted laser desorption ionization-time of flight mass spectrometry (DIT MALDI-TOF MS). Journal of Mass Spectrometry, v. 51, n. 8, p. 549-557, AUG 2016. (14/06863-3)

KAWAHARA, REBECA; SAAD, JOYCE; ANGELI, CLAUDIA BLANES; PALMISANO, GIUSEPPE. Site-specific characterization of N-linked glycosylation in human urinary glycoproteins and endogenous glycopeptides. GLYCOCONJUGATE JOURNAL, v. 33, n. 6, p. 937-951, DEC 2016. (15/02866-0, 14/06863-3)

KAWAHARA, REBECA; RECUERO, SAULO; SROUGI, MIGUEL; LEITE, KATIA R. M.; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. The Complexity and Dynamics of the Tissue Glycoproteome Associated With Prostate Cancer Progression. MOLECULAR & CELLULAR PROTEOMICS, v. 20, 2021. (14/06863-3, 15/02866-0, 17/03010-8, 18/15549-1, 18/18257-1)

AVILA, CARLA CRISTI; MULE, SIMON NGAO; ROSA-FERNANDES, LIVIA; VINER, ROSA; BARISON, MARIA JULIA; COSTA-MARTINS, ANDRE GUILLHERME; DE OLIVEIRA, GILBERTO SANTOS; GERALDES TEIXEIRA, MARTA MARIA; FARIAS MARINHO, CLAUDIO ROMERO; SILBER, ARIEL MARIANO; et al. Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation. GENES, v. 9, n. 8, AUG 2018. (16/06034-2, 14/06863-3)

KISCHKEL, BRENDA; BONICHE-ALFARO, CAMILA; MENEZES, ISABELA DE GODOY; ROSSI, SUELEN ANDREIA; ANGELI, CLAUDIA BLANES; DE ALMEIDA, SANDRO ROGERIO; PALMISANO, GIUSEPPE; LOPES-BEZERRA, LEILA; NOSANCHUK, JOSHUA D.; TABORDA, CARLOS PELLESCHI. Immunoproteomic and Immunopeptidomic Analyses of Histoplasma capsulatum Reveal Promiscuous and Conserved Epitopes Among Fungi With Vaccine Potential. FRONTIERS IN IMMUNOLOGY, v. 12, NOV 22 2021. (18/18257-1, 16/08730-6, 14/06863-3, 17/25780-0, 18/26402-1, 18/15549-1)

CARREGARI, VICTOR CORASSOLLA; ROSA-FERNANDES, LIVIA; BALDASSO, PAULO; BYDLOWSKI, SERGIO PAULO; MARANGONI, SERGIO; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Snake Venom Extracellular vesicles (SVEVs) reveal wide molecular and functional proteome diversity. SCIENTIFIC REPORTS, v. 8, AUG 13 2018. (14/06863-3)

ROSA-FERNANDES, LIVIA; CUGOLA, FERNANDA RODRIGUES; RUSSO, FABIELE BALDINO; KAWAHARA, REBECA; DE MELO FREIRE, CAIO CESAR; CORREA LEITE, PAULO EMLIO; BASSI STERN, ANA CAROLINA; ANGELI, CLAUDIA BLANES; LEAL DE OLIVEIRA, DANIELLE BRUNA; MELO, STELLA REZENDE; et al. Zika Virus Impairs Neurogenesis and Synaptogenesis Pathways in Human Neural Stem Cells and Neurons. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 13, MAR 15 2019. (16/02978-6, 14/06863-3, 13/08844-3, 18/16748-8, 11/20683-0, 15/02866-0)

TANO, FABIA TOMIE; BARBOSA, GUSTAVO ROLIM; DE REZENDE, ELOIZA; OLIVEIRA SOUZA, RODOLPHO ORNITZ; MUXEL, SANDRA MARCIA; SILBER, ARIEL MARIANO; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteome and morphological analysis show unexpected differences between promastigotes of Leishmania amazonensis PH8 and LV79 strains. PLoS One, v. 17, n. 8, p. 26-pg., 2022-08-23. (18/15549-1, 18/18257-1, 18/14972-8, 17/26197-6, 14/06863-3)

CARREGARI, VICTOR CORASSOLLA; ROSA-FERNANDES, LIVIA; BALDASSO, PAULO; BYDLOWSKI, SERGIO PAULO; MARANGONI, SERGIO; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Snake Venom Extracellular vesicles (SVEVs) reveal wide molecular and functional proteome diversity (vol 8, 15908,2018). SCIENTIFIC REPORTS, v. 8, p. 1-pg., 2018-10-23. (14/06863-3)

BOYSEN, ANDERS; PALMISANO, GIUSEPPE; KROGH, THOGER JENSEN; DUGGIN, IAIN G.; LARSEN, MARTIN R.; MOLLER-JENSEN, JAKOB. A novel mass spectrometric strategy "BEMAP" reveals Extensive O-linked protein glycosylation in Enterotoxigenic Escherichia coli. SCIENTIFIC REPORTS, v. 6, p. 13-pg., 2016-08-26. (14/06863-3)

FERNANDES, LIVIA ROSA; BASSI STERN, ANA CAROLINA; CAVAGLIERI, RITA DE CASSIA; SOUSA NOGUEIRA, FABIO CESAR; DOMONT, GILBERTO; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO PAULO. 7-Ketocholesterol overcomes drug resistance in chronic myeloid leukemia cell lines beyond MDRI mechanism. JOURNAL OF PROTEOMICS, v. 151, p. 12-pg., 2017-01-16. (14/06863-3)

MATTOS, ELICIANE CEVOLANI; PALMISANO, GIUSEPPE; GOLDMAN, GUSTAVO H.. Phosphoproteomics of Aspergillus fumigatus Exposed to the Antifungal Drug Caspofungin. MSPHERE, v. 5, n. 3, p. 16-pg., 2020-05-01. (18/15549-1, 18/18257-1, 17/19288-5, 16/07870-9, 14/06863-3)

ROSA-FERNANDES, LIVIA; MASELLI, LUCIANA M. P.; MAEDA, NAIR Y.; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO P.. Outside-in, inside-out: Proteomic analysis of endothelial stress mediated by 7-ketocholesterol. Chemistry and Physics of Lipids, v. 207, p. 8-pg., 2017-10-01. (14/06863-3)

ROSA-FERNANDES, LIVIA; ROCHA, VICTORIA BOMBARDA; CARREGARI, VICTOR CORASOLLA; URBANI, ANDREA; PALMISANO, GIUSEPPE. A Perspective on Extracellular Vesicles Proteomics. RONTIERS IN CHEMISTR, v. 5, p. 19-pg., 2017-11-21. (14/06863-3)

COUTINHO, JOAO V. P.; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; DE OLIVEIRA, GILBERTO SANTOS; MANCHOLA, NUBIA CAROLINA; SANTIAGO, VERONICA FEIJOLI; COLLI, WALTER; WRENGER, CARSTEN; MANSO ALVES, MARIA JULIA; PALMISANO, GIUSEPPE. The thermal proteome stability profile of Trypanosoma cruzi in epimastigote and trypomastigote life stages. JOURNAL OF PROTEOMICS, v. 248, SEP 30 2021. (18/13283-4, 18/15549-1, 14/06863-3, 17/03966-4, 15/26722-8, 17/19854-0, 17/04032-5, 18/18257-1)

HUANG, HONGGANG; DRICI, LYLIA; LASSEN, PERNILLE S.; PALMISANO, GIUSEPPE; LARSEN, MARTIN R.. TiCPG-a strategy for the simultaneous enrichment of reversibly modified cysteine peptides, phosphopeptides, and sialylated N-Glycopeptides to study cytokines stimulated beta-cells. JOURNAL OF PROTEOMICS, v. 273, p. 13-pg., 2022-12-28. (14/06863-3)

KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, SI, NOV 2019. (18/15549-1, 15/02866-0, 18/18257-1, 17/03010-8, 14/06863-3)

ROSA-FERNANDES, LIVIA; MASELLI, LUCIANA M. P.; MAEDA, NAIR Y.; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO P.. Outside-in, inside-out: Proteomic analysis of endothelial stress mediated by 7-ketocholesterol. Chemistry and Physics of Lipids, v. 207, n. B, p. 231-238, OCT 2017. (14/06863-3)

ROSA-FERNANDES, LIVIA; ROCHA, VICTORIA BOMBARDA; CARREGARI, VICTOR CORASOLLA; URBANI, ANDREA; PALMISANO, GIUSEPPE. A Perspective on Extracellular Vesicles Proteomics. FRONTIERS IN CHEMISTRY, v. 5, NOV 21 2017. (14/06863-3)

FERNANDES, LIVIA ROSA; BASSI STERN, ANA CAROLINA; CAVAGLIERI, RITA DE CASSIA; SOUSA NOGUEIRA, FABIO CESAR; DOMONT, GILBERTO; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO PAULO. 7-Ketocholesterol overcomes drug resistance in chronic myeloid leukemia cell lines beyond MDRI mechanism. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 12-23, JAN 16 2017. (14/06863-3)

DE OLIVEIRA, GILBERTO SANTOS; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; AVILA, CARLA C.; LARSEN, MARTIN R.; PEREIRA ALVES, JOAO MARCELO; PALMISANO, GIUSEPPE. Novel DNA coding regions and protein arginylation reveal unexplored T. cruzi proteome and PTMs. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 418, n. SI, p. 51-66, JUL 2017. (15/02866-0, 14/06863-3)

LIMA, GUILHERME MEIRA; EFFER, BRIAN; BIASOTO, HENRIQUE PELLIN; FEIJOLI, VERONICA; PESSOA, ADALBERTO; PALMISANO, GIUSEPPE; MONTEIRO, GISELE. Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis. Biochemical Engineering Journal, v. 156, APR 15 2020. (15/07749-2, 13/08617-7, 14/06863-3, 18/18257-1, 18/15549-1, 16/15787-4)

GAVINHO, BRUNO; SABATKE, BRUNA; FEIJOLI, VERONICA; ROSSI, IZADORA VOLPATO; DA SILVA, JANAINA MACEDO; EVANS-OSSES, INGRID; PALMISANO, GIUSEPPE; LANGE, SIGRUN; RAMIREZ, MARCEL IVAN. Peptidylarginine Deiminase Inhibition Abolishes the Production of Large Extracellular Vesicles FromGiardia intestinalis, Affecting Host-Pathogen Interactions by Hindering Adhesion to Host Cells. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, SEP 23 2020. (18/15549-1, 18/18257-1, 14/06863-3)

ROSA-FERNANDES, LIVIA; BARBOSA, RAQUEL HORA; DOS SANTOS, MARIA LUIZA B.; ANGELI, CLAUDIA B.; SILVA, THIAGO P.; MELO, ROSSANA C. N.; DE OLIVEIRA, GILBERTO SANTOS; LEMOS, BERNARDO; VAN EYK, JENNIFER E.; LARSEN, MARTIN R.; et al. Cellular Imprinting Proteomics Assay: A Novel Method for Detection of Neural and Ocular Disorders Applied to Congenital Zika Virus Syndrome. JOURNAL OF PROTEOME RESEARCH, v. 19, n. 11, p. 4496-4515, NOV 6 2020. (18/15549-1, 18/18257-1, 14/06863-3)

RODRIGUES, MARIANE A. D.; PIMENTA, MARCELA V.; COSTA, IRIS M.; ZENATTI, PRISCILA P.; MIGITA, NATACHA A.; YUNES, JOSE A.; RANGEL-YAGUI, CARLOTA O.; DE SA, MATHEUS M.; PESSOA, ADALBERTO; COSTA-SILVA, TALES A.; et al. Influence of lysosomal protease sensitivity in the immunogenicity of the antitumor biopharmaceutical asparaginase. Biochemical Pharmacology, v. 182, DEC 2020. (14/06863-3, 18/18257-1, 13/08617-7, 16/25896-5, 15/07749-2, 18/15104-0, 13/08139-8, 18/15549-1)

GOMES, LAURA CORDEIRO; SANSON, MARINA CORDEIRO GOMES; BRAININ, PHILIP; DE MELO, MARIA DA CONCEICAO VIEIRA; DE SOUZA, RODRIGO MEDEIROS; MAZARO, JANAINA; LIMA, KARINE OLIVEIRA; RESENDE, JUNIA SILVA; VIEIRA, ISABELLE VICTORIA MARTINS; MESQUITA, EDINILSON DA SILVA; et al. Levels of hepatitis B antibody titers are affected by age and doses gap time in children from a high endemic area of the western Amazon. PLoS One, v. 16, n. 7, 2021. (18/20468-0, 18/18257-1, 18/15549-1, 15/26722-8, 14/06863-3)

PEREIRA, ADRIANA S. A.; AMARAL, MURILO S.; VASCONCELOS, ELTON J. R.; PIRES, DAVID S.; ASIF, HUMA; DASILVA, LUCAS F.; MORALES-VICENTE, DAVID A.; CARNEIRO, VITOR C.; ANGELI, CLAUDIA B.; PALMISANO, GIUSEPPE; et al. Inhibition of histone methyltransferase EZH2 in Schistosoma mansoni in vitro by GSK343 reduces egg laying and decreases the expression of genes implicated in DNA replication and noncoding RNA metabolism. PLoS Neglected Tropical Diseases, v. 12, n. 10, OCT 2018. (16/10046-6, 14/24560-8, 14/06863-3)

FUZITA, FELIPE J.; PIMENTA, DANIEL C.; PALMISANO, GIUSEPPE; TERRA, WALTER R.; FERREIRA, CLELIA. Detergent-resistant domains in Spodoptera frugiperda midgut microvillar membranes and their relation to microapocrine secretion. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 235, p. 8-18, SEP 2019. (17/08103-4, 14/14183-2, 14/06863-3)

RAMIREZ, MARCEL I.; AMORIM, MARIA G.; GADELHA, CATARINA; MILIC, IVANA; WELSH, JOSHUA A.; FREITAS, VANESSA M.; NAWAZ, MUHAMMAD; AKBAR, NAVEED; COUCH, YVONNE; MAKIN, LAURA; et al. Technical challenges of working with extracellular vesicles. NANOSCALE, v. 10, n. 3, p. 881-906, JAN 21 2018. (14/26897-0, 12/24574-3, 14/06863-3, 15/50257-3)

MOREIRA, RAFAEL J.; CASTRO, ERIQUE; OLIVEIRA, TIAGO E.; BELCHIOR, THIAGO; PEIXOTO, ALBERT S.; CHAVES-FILHO, ADRIANO B.; MORENO, MAYARA F.; LIMA, JANAYNA D.; YOSHINAGA, MARCOS; MIYAMOTO, SAYURI; et al. Lipoatrophy-Associated Insulin Resistance and Hepatic Steatosis are Attenuated by Intake of Diet Rich in Omega 3 Fatty Acids. MOLECULAR NUTRITION & FOOD RESEARCH, v. 64, n. 7, FEB 2020. (13/07467-1, 16/04000-3, 17/17943-6, 15/19530-5, 14/06863-3, 18/15549-1, 13/07937-8)

DE CASTRO, PATRICIA ALVES; COLABARDINI, ANA CRISTINA; MANFIOLLI, ADRIANA OLIVEIRA; CHIARATTO, JESSICA; SILVA, LILIAN PEREIRA; MATTOS, ELICIANE CEVOLANI; PALMISANO, GIUSEPPE; ALMEIDA, FAUSTO; PERSINOTI, GABRIELA FELIX; ANNICK RIES, LAURE NICOLAS; et al. Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance. PLOS GENETICS, v. 15, n. 12, DEC 2019. (17/07536-4, 16/21392-2, 14/06863-3, 16/12948-7, 16/07870-9, 16/22062-6, 14/04783-2, 17/19288-5)

HUANG, HONGGANG; PETERSEN, MARTIN HAAR; IBANEZ-VEA, MARIA; LASSEN, PERNILLE S.; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. MOLECULAR & CELLULAR PROTEOMICS, v. 15, n. 10, p. 3282-3296, OCT 2016. (14/06863-3)

CIREGIA, FEDERICA; URBANI, ANDREA; PALMISANO, GIUSEPPE. Extracellular Vesicles in Brain Tumors and Neurodegenerative Diseases. FRONTIERS IN MOLECULAR NEUROSCIENCE, v. 10, AUG 31 2017. (16/50356-4, 14/06863-3)

KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, p. 12-pg., 2019-10-20. (17/03010-8, 18/18257-1, 18/15549-1, 14/06863-3, 15/02866-0)

LATROFA, MARIA STEFANIA; PALMISANO, GIUSEPPE; ANNOSCIA, GIADA; PIERRI, CIRO LEONARDO; CHANDRASHEKAR, RAMASWAMY; OTRANTO, DOMENICO. Major antigen and paramyosin proteins as candidate biomarkers for serodiagnosis of canine infection by zoonotic Onchocerca lupi. PLoS Neglected Tropical Diseases, v. 15, n. 2, FEB 2021. (17/04032-5, 18/18257-1, 14/06863-3, 18/15549-1)

MACEDO-DA-SILVA, JANAINA; MARINHO, CLAUDIO ROMERO FARIAS; PALMISANO, GIUSEPPE; ROSA-FERNANDES, LIVIA. Lights and Shadows of TORCH Infection Proteomics. GENES, v. 11, n. 8, p. 31-pg., 2020-08-01. (14/06863-3, 18/20468-0, 18/18257-1, 18/15549-1)

CHATTERJEE, SAYANTANI; LEE, LING Y.; KAWAHARA, REBECA; ABRAHAMS, JODIE L.; ADAMCZYK, BARBARA; ANUGRAHAM, MERRINA; ASHWOOD, CHRISTOPHER; SUMER-BAYRAKTAR, ZEYNEP; BRIGGS, MATTHEW T.; CHIK, JENNY H. L.; et al. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. PROTEOMICS, v. 19, n. 21-22, p. 13-pg., 2019-10-16. (18/15549-1, 17/03010-8, 14/06863-3, 15/02866-0)

MANSO ALVES, MARIA JULIA; KAWAHARA, REBECA; VINER, ROSA; COLLI, WALTER; MATTOS, ELICIANE CEVOLANI; THAYSEN-ANDERSEN, MORTEN; LARSEN, MARTIN ROSSEL; PALMISANO, GIUSEPPE. Comprehensive glycoprofiling of the epimastigote and trypomastigote stages of Trypanosoma cruzi. JOURNAL OF PROTEOMICS, v. 151, p. 11-pg., 2017-01-16. (14/25494-9, 13/16478-7, 14/06863-3)

DE OLIVEIRA, GILBERTO SANTOS; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; AVILA, CARLA C.; LARSEN, MARTIN R.; PEREIRA ALVES, JOAO MARCELO; PALMISANO, GIUSEPPE. Novel DNA coding regions and protein arginylation reveal unexplored T. cruzi proteome and PTMs. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 418, p. 16-pg., 2017-07-01. (14/06863-3, 15/02866-0)

ROSA-FERNANDES, LIVIA; BEDRAT, AMINA; DOS SANTOS, MARIA LUIZA B.; PINTO, ANA M., V; LUCENA, EVANDRO; SILVA, THIAGO P.; MELO, ROSSANA C. N.; PALMISANO, GIUSEPPE; CARDOSO, CLAUDETE ARAUJO; BARBOSA, RAQUEL HORA. Global RNAseq of ocular cells reveals gene dysregulation in both asymptomatic and with Congenital Zika Syndrome infants exposed prenatally to Zika virus. Experimental Cell Research, v. 414, n. 2, p. 16-pg., 2022-05-15. (14/06863-3)

MISERANI MAGALHAES, RUBENS DANIEL; MATTOS, ELICIANE CEVOLANI; ROZANSKI, ANDREI; FAVORETTO GALANTE, PEDRO ALEXANDRE; PALMISANO, GIUSEPPE; CRUZ, ANGELA KAYSEL; COLLI, WALTER; CAMARGO, ANAMARIA ARANHA; MANSO ALVES, MARIA JULIA. Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix. PLoS Neglected Tropical Diseases, v. 14, n. 5, MAY 2020. (14/10046-0, 13/16002-2, 18/15549-1, 18/18257-1, 14/25494-9, 14/06863-3)

TROMBETTA-LIMA, MARINA; ROSA-FERNANDES, LIVIA; ANGELI, CLAUDIA B.; MORETTI, ISABELE F.; FRANCO, YOLLANDA M.; MOUSESSIAN, ADALIANA S.; WAKAMATSU, ALDA; LERARIO, ANTONIO M.; OBA-SHINJO, SUELI M.; PASQUALUCCI, CARLOS A.; et al. Extracellular Matrix Proteome Remodeling in Human Glioblastoma and Medulloblastoma. JOURNAL OF PROTEOME RESEARCH, v. 20, n. 10, p. 4693-4707, OCT 1 2021. (14/06863-3, 16/14695-9, 15/26328-8)

MULE, SIMON NGAO; COSTA-MARTINS, ANDRE GUILHERME; ROSA-FERNANDES, LIVIA; DE OLIVEIRA, GILBERTO SANTOS; RODRIGUES, CARLA MONADELI F.; QUINA, DANIEL; ROSEIN, GRAZIELLA E.; GERALDES TEIXEIRA, MARTA MARIA; PALMISANO, GIUSEPPE. PhyloQuant approach provides insights into Trypanosoma cruzi evolution using a systems-wide mass spectrometry-based quantitative protein profile. COMMUNICATIONS BIOLOGY, v. 4, n. 1, MAR 11 2021. (18/13283-4, 17/04032-5, 14/06863-3, 18/15549-1, 18/18257-1, 14/25494-9)

FUZITA, FELIPE J.; PALMISANO, GIUSEPPE; PIMENTA, DANIEL C.; TERRA, WALTER R.; FERREIRA, CLELIA. A proteomic approach to identify digestive enzymes, their exocytic and microapocrine secretory routes and their compartmentalization in the midgut of Spodoptera frugiperda. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 257, JAN 2022. (14/06863-3, 17/08103-4, 14/14183-2)