Investigação dos efeitos dos herbicidas trifluralina e Tebutiurom na ativação da via de sinalização de defesa ao estresse oxidativo Keap1/Nrf2- are

• Processo: 14/02170-3
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Vigência (Início): 01 de outubro de 2014
• Vigência (Término): 31 de março de 2015
• Área do conhecimento: Ciências Biológicas - Farmacologia - Toxicologia
• Pesquisador responsável: Daniel Junqueira Dorta
• Beneficiário: Mariana Furio Franco Bernardes
• Supervisor: Kendall B. Wallace
• Instituição Sede: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brasil
• Local de pesquisa: University of Minnesota Duluth (UMD), Estados Unidos
• Vinculado à bolsa: 12/15220-3 - Avaliação da citotoxicidade, genotoxicidade e mutagenicidade dos herbicidas tebutiurom e trifluralina e de seus efeitos na expressão de genes de resposta ao estresse celular, BP.DR
• Palavra(s)-Chave do Pesquisador: antioxidant response element | Keap1 | Nrf2-ARE | Oxidative stress | Rt Pcr | Western blot | Ensaios toxicológicos

Resumo

The word "pesticide" covers any substance or mixture of substances used for preventing, destroying, repelling or mitigating pests, or intended for use as plant regulators, defoliants or dessicants. One of the most common types of pesticides being used are herbicides, a heterogeneous category of chemical products, designed to control weeds. These xenobiotics can be grouped according to the mechanism of action in plants. Trifluralin belongs to dinitroanilines chemical group and its mechanism of action is by inhibition of cell division by inhibiting microtubule formation. It is used, among other crops, in soybean plantations. Tebuthiuron belongs to substituted ureas chemical group and its mechanism of action is by inhibition of photosystem II of photosynthesis. It is widely used in sugar cane crops. Currently, herbicides play an important role in controlling weeds, however, most cost-benefit analyses do not consider the environmental, ecological, and public health costs associated with use of herbicides. Although some herbicides are described as selective in their mechanism of action, their range selectivity is limited in some animals tested. Thus, the study of these compounds is extremely important. In order to investigate the redox regulation of protein-mediated transcriptional activation of the NRF2 directed translocation and binding to the antioxidant response element, the techniques of western blots and real time PCR will be used. These techniques are well established in the laboratory of Prof. Dr. Kendall B. Wallace and will be will be extremely important to add knowledge to studies with herbicides trifluralin and tebuthiuron. (AU)