The Na/H exchanger isoform 3 (NHE3) is present in the apical membrane of the renal proximal tubule and mediates the majority of NaCl and NaHCO3 reabsorption in this nephron segment. Given its importance in body fluid homeostasis, NHE3 is tightly regulated by a broad range of effectors via multiple molecular mechanisms. Rapid regulation of NHE3 activity may occur through subcellular redistribution of the transporter between the microdomains of the proximal tubule (PT) microvilli (MV). However, the mechanisms involved in the redistribution of NHE3 in the MV remain unclear. Since myosin IIA directly binds to NHE3 in the renal proximal tubule and is involved in the protein trafficking, we will test the hypothesis that myosin IIA motor protein translocates NHE3 from the intermicrovilar to the microvillar microdomain in the proximal tubule when there is a call for increased Na+ reabsorption.
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