Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Activation of spleen cells by ArtinM may account for its immunomodulatory properties

Texto completo
Autor(es):
da Silva, Thiago Aparecido [1] ; de Souza, Maria Aparecida [1] ; Cecilio, Nerry Tatiana [1] ; Roque-Barreira, Maria Cristina [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Biol Celular & Mol Bioagentes Patogen, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Cell and Tissue Research; v. 357, n. 3, p. 719-730, SEP 2014.
Citações Web of Science: 13
Resumo

ArtinM is a D-mannose-binding lectin extracted from Artocarpus heterophyllus that promotes interleukin-12 production by macrophages and dendritic cells. This property is considered responsible for T helper 1 immunity induced in vivo after ArtinM administration. In this study, we investigated the effect of native (jArtinM) and recombinant (rArtinM) forms of lectin on murine spleen cells and isolated T lymphocytes. We found that ArtinM binds to the surface of spleen cells. This interaction, which was blocked by D-mannose, induced cell activation, as manifested by increased mitochondrial activity, interleukin-2 production, and cell proliferation. We verified that a 30-times higher concentration of rArtinM was required to trigger optimal activation of spleen cells compared with that needed with jArtinM, although these proteins have identical sugar recognition properties and use the same signaling molecules to trigger cell activation. Because the distinction between native and recombinant is restricted to their tertiary structure (tetrameric and monomeric, respectively), we postulated that the multi-valence of jArtinM accounts for its superiority in promoting clustering of cell surface glycoreceptors and activation. The jArtinM and rArtinM activation effect exerted on spleen cells was reproduced on purified CD4(+) T cells. Our results suggest that ArtinM interaction with T cells leads to responses that may act in concert with the interleukin-12 produced by antigen-presenting cells to modulate immunity toward the T helper 1 axis. Further studies are necessary to dissect ArtinM/T-cell interactions to more fully understand the immunomodulation induced by carbohydrate recognition. (AU)

Processo FAPESP: 10/51763-6 - Maria Aparecida de Souza | Univ. Federal de Uberlandia / UFU - Brasil
Beneficiário:Maria Cristina Roque Antunes Barreira
Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Brasil
Processo FAPESP: 12/09611-0 - Efeito da lectina ArtinM sobre as células T CD4+ e T CD8+ murinas
Beneficiário:Thiago Aparecido da Silva
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 06/60642-2 - Efeitos biológicos e aplicações farmacêuticas de lectinas
Beneficiário:Maria Cristina Roque Antunes Barreira
Linha de fomento: Auxílio à Pesquisa - Temático