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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Xanthomonas citri subsp citri Type IV Pilus Is Required for Twitching Motility, Biofilm Development, and Adherence

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Autor(es):
Dunger, German [1] ; Guzzo, Cristiane R. [1, 2] ; Andrade, Maxuel O. [1] ; Jones, Jeffrey B. [3] ; Farah, Chuck S. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, BR-05508000 Sao Paulo - Brazil
[3] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 27, n. 10, p. 1132-1147, OCT 2014.
Citações Web of Science: 24
Resumo

Bacterial type IV pili (T4P) are long, flexible surface filaments that consist of helical polymers of mostly pilin subunits. Cycles of polymerization, attachment, and depolymerization mediate several pilus-dependent bacterial behaviors, including twitching motility, surface adhesion, pathogenicity, natural transformation, escape from immune system defense mechanisms, and biofilm formation. The Xanthomonas citri subsp. citri strain 306 genome codes for a large set of genes involved in T4P biogenesis and regulation and includes several pilin homologs. We show that X. citri subsp. citri can exhibit twitching motility in a manner similar to that observed in other bacteria such as Pseudomonas aeruginosa and Xylella fastidiosa and that this motility is abolished in Xanthomonas citri subsp. citri knockout strains in the genes coding for the major pilin subunit PilA(XAC3241), the ATPases PilB(XAC3239) and PilT(XAC2924), and the T4P biogenesis regulators PilZ(XAC1133) and FiMX(XAC2398). Microscopy analyses were performed to compare patterns of bacterial migration in the wild-type and knockout strains and we observed that the formation of mushroom-like structures in X. citri subsp. citri biofilm requires a functional T4P. Finally, infection of X. citri subsp. citri cells by the bacteriophage Phi Xacm4-11 is T4P dependent. The results of this study improve our understanding of how T4P influence Xanthomonas motility, biofilm formation, and susceptibility to phage infection. (AU)

Processo FAPESP: 11/22571-4 - Regulação do pilus Tipo IV de Xanthomonas citri
Beneficiário:Ricardo Germán Dunger
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/07777-5 - Sinalização por c-di-GMP e o sistema de secreção de macromoléculas do tipo IV em Xanthomonas citri
Beneficiário:Shaker Chuck Farah
Linha de fomento: Auxílio à Pesquisa - Temático