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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Short-term dietary magnesium restriction lowers spleen iron concentrations in growing rats fed a high-fat diet

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Autor(es):
De Carli, Eduardo [1] ; Lobo, Alexandre R. [1] ; Sales, Cristiane H. [1] ; Teixeira, Pryscila D. S. [1] ; Sales, Ana Lina de Carvalho C. [1] ; Colli, Celia [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, BR-05508900 Sao Paulo, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: LWT-FOOD SCIENCE AND TECHNOLOGY; v. 59, n. 2, 2, SI, p. 1298-1303, DEC 2014.
Citações Web of Science: 0
Resumo

Lipotoxicity and dietary inadequacies have been associated with dysregulation of Fe homeostasis in obesity. In this study, we tested the hypothesis that dietary Mg restriction in rats fed a high-fat diet affects Fe concentrations in liver and spleen, tissues related with Fe storage and recycling, respectively. Weanling male Wistar rats (n = 48) were fed either control (ad libitum or pair-fed) or high-fat (Mg-adequate or Mg-restricted; 500 and 150 mg Mg/kg, respectively) diets for 4 weeks. Epididymal and retroperitoneal fat pad weights were used to determine the adiposity index. Serum (colorimetry) and tissue Fe (atomic absorption spectrophotometry) were analyzed. Liver hepcidin was determined by qPCR and ferroportin-1 in liver and spleen was measured by immunoblotting. Dietary fat overload increased adiposity irrespective of dietary Mg levels. Rats fed the high-fat diet, but not those fed the high-fat Mg-restricted diet, had higher spleen Fe concentrations than controls. Weak but significant associations between body adiposity and serum (r = -0.31, P = 0.04) and spleen Fe (r = 0.29, P = 0.04) concentrations were obtained. The unaltered ferroportin-1 expression with low Fe levels suggests increased Fe recycling from spleen to bone marrow due to dietary Mg restriction. A longer study could clarify the consequences of these findings on erythropoiesis and other conditions related to diet-induced obesity. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 09/05624-7 - Efeito da deficiência dietética de magnésio na resistência insulínica e na resposta inflamatória em ratos alimentados com dieta hiperlipídica
Beneficiário:Cristiane Hermes Sales
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/08895-1 - Status de ferro em ratos alimentados com rações hiperlipídicas e deficientes em magnésio: contribuição da inflamação sistêmica e do microambiente intestinal
Beneficiário:Alexandre Rodrigues Lobo
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/05644-5 - Avaliação do status de ferro em ratos alimentados com rações hiperlipídicas
Beneficiário:Célia Colli
Linha de fomento: Auxílio à Pesquisa - Regular