Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response

Autor(es)

Silva, Carla C. ^[1] ; Mantello, Camila C. ^[1] ; Campos, Tatiana ^[2] ; Souza, Livia M. ^[1] ; Goncalves, Paulo S. ^[3] ; Souza, Anete P. ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet CBMEG, BR-13083970 Campinas, SP - Brazil

^[2] EMBRAPA, Ctr Pesquisa Agroflorestal Acre CPAFAC, BR-69900970 Rio Branco 69900970, Ac - Brazil

^[3] Inst Agronm Campinas IAC, BR-13012970 Campinas, SP - Brazil

^[4] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BREEDING; v. 34, n. 3, p. 1035-1053, OCT 2014.
• Citações Web of Science: 11

Resumo

Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early evaluation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasiliensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. (AU)

• Processo FAPESP: 07/50562-4 - Construção de um mapa genético-molecular com microssatélites e mapeamento de locos ligados à tolerância ao frio e outras características de importância econômica em seringueira
• Beneficiário: Anete Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/50491-8 - Avaliação da variabilidade alélica, estrutura populacional e desequilíbrio de ligação no germoplasma ex situ de seringueira (Hevea brasiliensis)
• Beneficiário: Anete Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50188-0 - Mapeamento genético molecular em Hevea brasiliensis
• Beneficiário: Camila Campos Mantello
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/52975-0 - Construção de um mapa funcional em seringueira (Hevea brasiliensis)
• Beneficiário: Carla Cristina da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/05473-1 - Avaliação do desequilíbrio de ligação e estrutura populacional no germoplasma ex situ de seringueira (Hevea brasiliensis) através de marcadores moleculares SNP (Single Nucleotide Polymorphism)
• Beneficiário: Livia Moura de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/55974-1 - Mapeamento genético molecular de Hevea brasiliensis
• Beneficiário: Camila Campos Mantello
• Modalidade de apoio: Bolsas no Brasil - Mestrado