Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients

Autor(es)

Ribeiro de Noronha, Samuel Marcos ^[1] ; Alves Correa de Noronha, Silvana Aparecida ^[1] ; Klepp, Anthony Gueratto ^[2] ; Ipolito, Michele Zampieri ^[3] ; Ferreira, Lydia Masako ^[1] ; Gragnani, Alfredo ^[4]

Afiliação do(s) autor(es)

^[1] UNIFESP EPM, Dept Surg, Div Plast Surg, Sao Paulo - Brazil

^[2] UNIFESP EPM, Sao Paulo - Brazil

^[3] UNIFESP EPM, Dept Gynecol, Lab Mol Gynecol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Paulista Sch Med UNIFESP EPM, Dept Surg, Div Plast Surg, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 29, n. 1, p. 62-68, 2014.
• Citações Web of Science: 2

Resumo

Purpose: To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn. Methods: Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supernatant of the culture medium was measured by, respectively, flow cytometry using Cytometric Bead Array Human Inflammation kit (CBA, BD Biosciences, USA) and the enzyme immunoassay method using the Quantikine (R) Human KGF. The experiments were performed in triplicate. Results: The expression of IL-12 protein in patients with large burns showed a tendency to increase. IL-6, IL-10, and IL-1beta were observed no difference. For IL - 8, TNF - alpha and KGF was observed a significant difference between the expression in large and small burned patient. Conclusion: That IL-8, TNF-alpha and KGF showed higher expression in cultured fibroblasts of large burned patients. (AU)

• Processo FAPESP: 11/12945-4 - Fator de crescimento de queratinócitos, interleucinas 1 beta, 6, 8, 10, 12 e fator de necrose tumoral alfa em pacientes com queimadura
• Beneficiário: Alfredo Gragnani Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular