Investigation of DMSO-Induced Conformational Transitions in Human Serum Albumin Using Two-Dimensional Raman Optical Activity Spectroscopy

Autor(es)

Batista, Andrea N. L. ^{[1, 2, 3]} ; Batista, Jr., Joao M. ^{[1, 2, 3]} ; Ashton, Lorna ^{[2, 4]} ; Bolzani, Vanderlan S. ^[1] ; Furlan, Maysa ^[1] ; Blanch, Ewan W. ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Quim, Dept Quim Organ, BR-14800060 Araraquara, SP - Brazil

^[2] Univ Manchester, Manchester Inst Biotechnol, Manchester, Lancs - England

^[3] Univ Manchester, Fac Life Sci, Manchester, Lancs - England

^[4] Univ Manchester, Sch Chem, Manchester, Lancs - England

• Tipo de documento: Artigo Científico
• Fonte: Chirality; v. 26, n. 9, SI, p. 497-501, SEP 2014.
• Citações Web of Science: 10

Resumo

Recent Raman and Raman optical activity (ROA) results have demonstrated that dimethyl sulfoxide (DMSO) induces the selective conversion of alpha-helix motifs into the poly(L-proline) II (PPII) helix conformation in an array of proteins, while beta-sheets remain mostly unaffected. Human serum albumin (HSA), a highly alpha-helical protein, underwent the most dramatic changes and, therefore, was selected as a model for further investigations into the mechanism of this conformational change. Herein we report the use of two-dimensional ROA correlation analysis applying synchronous, autocorrelation, and moving windows approaches in order to understand the conformational transitions in HSA as a function of DMSO concentration. Our results indicate that the destabilization of native alpha-helix starts at DMSO concentrations as little as 20% in water (v/v), with the transition to PPII helix being complete at similar to 80% DMSO. These results clearly indicate that any protein preparation containing relatively low concentrations of DMSO should consider possible disruptions in alpha-helical domains. (C) 2014 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 12/16484-4 - Atividade óptica Raman: uma ferramenta inovadora para análise estrutural de proteínas
• Beneficiário: Andrea Nastri de Luca Batista
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 12/13739-1 - Atividade óptica vibracional no estudo da quiralidade molecular
• Beneficiário: João Marcos Batista Junior
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs