Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice

Texto completo
Autor(es):
Mostrar menos -
Ortolan, Luana S. [1, 2] ; Sercundes, Michelle K. [3] ; Barboza, Renato [4] ; Debone, Daniela [3] ; Murillo, Oscar [5] ; Hagen, Stefano C. F. [6] ; Russo, Momtchilo [1] ; D' Imperio Lima, Maria Regina [1] ; Alvarez, Jose M. [1] ; Amaku, Marcos [7] ; Marinho, Claudio R. F. [5] ; Epiphanio, Sabrina [1, 8]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Ciencias Biol, BR-09972270 Diadema, SP - Brazil
[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, BR-05403000 Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP - Brazil
[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508000 Sao Paulo - Brazil
[6] Univ Sao Paulo, Fac Med Vet & Zootecn, Dept Cirurgia, BR-05508270 Sao Paulo - Brazil
[7] Univ Sao Paulo, Fac Med Vet & Zootecn, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil
[8] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: Mediators of Inflammation; 2014.
Citações Web of Science: 6
Resumo

Malaria-associated acute lung injury/acute respiratory distress syndrome (ALI/ARDS) often results in morbidity and mortality. Murine models to study malaria-associated ALI/ARDS have been described; we still lack a method of distinguishing which mice will develop ALI/ARDS before death. This work aimed to characterize malaria-associated ALI/ARDS in a murine model and to demonstrate the first method to predict whether mice are suffering from ALI/ARDS before death. DBA/2 mice infected with Plasmodium berghei ANKA developing ALI/ARDS or hyperparasitemia (HP) were compared using histopathology, PaO2 measurement, pulmonary X-ray, breathing capacity, lung permeability, and serum vascular endothelial growth factor (VEGF) levels according to either the day of death or the suggested predictive criteria. We proposed a model to predict malaria-associated ALI/ARDS using breathing patterns (enhanced pause and frequency respiration) and parasitemia as predictive criteria from mice whose cause of death was known to retrospectively diagnose the sacrificed mice as likely to die of ALI/ARDS as early as 7 days after infection. Using this method, we showed increased VEGF levels and increased lung permeability in mice predicted to die of ALI/ARDS. This proposed method for accurately identifying mice suffering from ALI/ARDS before death will enable the use of this model to study the pathogenesis of this disease. (AU)

Processo FAPESP: 09/53889-0 - Estudo dos mecanismos imunopatológicos envolvidos na malária durante a gravidez
Beneficiário:Cláudio Romero Farias Marinho
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 09/53256-7 - Identificação e caracterização da síndrome respiratória aguda murina associada à malária severa: estudo da interação parasito-hospedeiro
Beneficiário:Sabrina Epiphanio
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores