Purification, crystallization and preliminary X-ray diffraction analysis of pyridoxal kinase from Plasmodium falciparum (PfPdxK)

Autor(es)

Kronenberger, Thales ^[1] ; Lunev, Sergey ^[2] ; Wrenger, Carsten ^[1] ; Groves, Matthew R. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Unit Drug Discovery, BR-05508000 Sao Paulo - Brazil

^[2] Univ Groningen, Dept Drug Design, Groningen Res Inst Pharm GRIP, NL-9713 AV Groningen - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 70, n. 11, p. 1550-1555, NOV 2014.
• Citações Web of Science: 0

Resumo

Pyridoxal kinases (PdxK) catalyze the phosphorylation of vitamin B-6 precursors. Thus, these enzymes are an essential part of many metabolic processes in all organisms. The protozoan parasite Plasmodium falciparum (the main causative agent of Malaria tropica) possesses a unique de novo B-6-biosynthesis pathway in addition to a interconversion pathway based on the activity of plasmodial PdxK (PfPdxK). The role of PdxK in B-6 salvage has prompted previous authors to suggest PdxK as a promising target for structure-based antimalarial drug design. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis of PfPdxK are reported. PfPdxK crystals have been grown in space group P2(1), with unit-cell parameters a = 52.7, b = 62.0, c = 93.7 angstrom, beta = 95 degrees. A data set has been collected to 2 angstrom resolution and an initial molecular-replacement solution is described. (AU)

• Processo FAPESP: 11/13706-3 - Implicações estruturais de mutantes da piridoxina quinase de Plasmodium falciparum e investigação das suas regiões espaçadoras
• Beneficiário: Thales Kronenberger
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/10288-1 - Análise da biogênese de organelas em Plasmodium falciparum por visualização celular em tempo real
• Beneficiário: Carsten Wrenger
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54325-2 - Elucidation of vitamin B metabolism in the human malaria parasite Plasmodium falciparum and their validation as a target for chemotherapy
• Beneficiário: Carsten Wrenger
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores