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N-acetylcysteine prevents endoplasmic reticulum stress elicited in macrophages by serum albumin drawn from chronic kidney disease rats and selectively affects lipid transporters, ABCA-1 and ABCG-1

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Autor(es):
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Machado, Juliana T. [1] ; Iborra, Rodrigo T. [1] ; Fusco, Fernanda B. [1] ; Castilho, Gabriela [1] ; Pinto, Raphael S. [1] ; Machado-Lima, Adriana [1] ; Nakandakare, Edna R. [1] ; Seguro, Antonio C. [2] ; Shimizu, Maria H. [2] ; Catanozi, Sergio [1] ; Passarelli, Marisa [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Sci, Lipids Lab LIM 10, Sch Med, BR-01246000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med Sci, Lab Basic Sci LIM 12, Sch Med, BR-01246000 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ATHEROSCLEROSIS; v. 237, n. 1, p. 343-352, NOV 2014.
Citações Web of Science: 11
Resumo

In chronic kidney disease (CKD) nontraditional risk factors, such as oxidative stress and advanced glycation end products (AGE) contribute to cardiovascular disease. Particularly, disturbances in reverse cholesterol transport favor the development of atherosclerosis. We analyzed the influence of N-acetylcysteine (NAC) in CKD rats on plasma concentration of lipid peroxides (TBARS) and AGE and on the impact of serum albumin in the development of macrophage endoplasmic reticulum stress (ERS) and cholesterol efflux, namely apo A-I and HDL2-mediated cholesterol removal and ABCA-1 and ABCG-1 protein level. CKD was induced by 5/6 nephrectomy in 2-month old male Wistar rats. Controls (Sham) were false operated. Animals were treated or not with NAC (600 mg/L of water). After 60 days serum albumin was isolated by FPLC and purified by alcoholic extraction. J774 macrophages were incubated with serum albumin (1 mg/mL; 18 h) from all groups, and the expression of ERS markers (protein disulfide isomerase - PDI, Grp78 and Grp94), ABCA-1 and ABCG-1 determined by immunoblot. HDL2 or apo A-I were used for cholesterol efflux assays. Protein and lipid composition of total HDL from Sham and CKD was determined and these particles tested on their abilities to accept cell cholesterol. Comparisons were done by one-way ANOVA and Newman Keuls post test. After 60 days of CKD, body weight was 10% lower in CKD compared to Sham (p < 0.01). This was prevented by NAC. Urea, creatinine, total cholesterol (TC), triglycerides (TG) (mg/dL), proteinuria (mg/24 h) (Sham, n = 31; Sham + NAC, n = 20; CKD, n = 74; CKD + NAC, n = 32), total AGE and pentosidine (n = 8; fluorescence arbitrary unit) and TBARS (n = 7; nmoL/mL) were higher in CKD (122 +/- 8; 0.9 +/- 0.07; 151 +/- 6; 83 +/- 4; 46 +/- 2.5; 32,620 +/- 673; 16,700 +/- 1,370; 6.6 +/- 0.5, respectively) and in CKD + NAC (91.4 +/- 5; 0.6 +/- 0.02; 126 +/- 7.5; 73 +/- 6; 51 +/- 3.5; 24,720 +/- 1,114; 10,080 +/- 748; 4.5 +/- 0.5, respectively) in comparison to Sham (41 +/- 0.9; 0.4 +/- 0.03; 76 +/- 2.7; 51.5 +/- 3; 14 +/- 0.9; 21,750 +/- 960; 5,314 +/- 129; 2.0 +/- 0.2, respectively; p < 0.001) and Sham + NAC (40 +/- 0.9; 0.3 +/- 0.02; 76 +/- 2.6; 68 +/- 4; 18.4 +/- 1.5; 20,040 +/- 700; 5,050 +/- 267; 1.8 +/- 0.2, respectively; p < 0.001). TC, urea, creatinine, total AGE, pentosidine and TBARS were respectively, 17%, 25%, 33%, 24%, 40% and 28% (p < 0.01) lower in CKD + NAC, than in CKD. Glycemia was higher in Sham + NAC (107 +/- 4.6) and CKD + NAC (107 +/- 2.6) than in Sham (96 +/- 1.8; p < 0.05) and CKD (98 +/- 1.6; p < 0.01), respectively. In macrophages (n = 6), CKD albumin increased PDI (3 and 6 times, p < 0.01) and Grp94 (66% and 80%, p < 0.01) in comparison to Sham and CKD + NAC-albumin treated cells, respectively. ABCA-1 expression was lower (87% and 70%, p < 0.001) in macrophage treated with Sham + NAC and CKD albumin respectively in comparison to Sham albumin; ABCG-1 was higher (4 and 7 times, p < 0.001) in macrophages treated with Sham + NAC and CKD + NAC albumin, respectively in comparison to Sham and CKD albumin. Apo A-I mediated cholesterol efflux was lower (59% and 70%, p < 0.0001) in macrophage treated with Sham + NAC and CKD albumin respectively in comparison to Sham albumin, however, the HDL2 mediated cholesterol efflux was higher (54% and 25%, p < 0. 0001) in macrophage treated with Sham + NAC albumin, in comparison to Sham and CKD + NAC albumin, respectively. CKD-HDL was enriched in total protein and lipids compared to Sham-HDL but preserved its capacity to remove cholesterol from macrophages. NAC reduces plasma lipid peroxidation and AGE and abrogates ERS induced by CKD-albumin. Despite diminishing ABCA-1, NAC increases ABCG-1 that counteracts the reduction in apo A-I-mediated cholesterol efflux. NAC may contribute to attenuate the deleterious effects of CKD-albumin on lipid accumulation in macrophages helping to prevent atherogenesis in CKD. (C) 2014 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 10/50108-4 - Glicoxidação intracelular e estresse do retículo endoplasmático: efeito potenciais sobre os transportadores ABCA-1 e sobre o fluxo de lípides em macrófagos
Beneficiário:Gabriela Castilho
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 09/52152-3 - Influência da modificação da HDL e da albumina em modelo experimental de uremia sobre o transporte reverso de colesterol de macrófagos
Beneficiário:Juliana Tironi Machado
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 06/55496-7 - Influência da glicoxidação sobre a expressão diferencial de genes macrófagos e sobre o fluxo de lípides, mediado por HDL
Beneficiário:Adriana Machado Saldiba de Lima
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 10/50147-0 - Albumina modificada por glicação avançada sensibiliza macrófagos a inflamação prejudicando o transporte reverso de colesterol e a atividade anti-inflamatória da HDL
Beneficiário:Ligia Shimabukuro Okuda
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 07/57745-7 - Influência dos produtos avançados de glicação (age) sobre o fluxo de lípides celulares em modelo experimental de uremia
Beneficiário:Marisa Passarelli
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/54688-8 - Estresse glicoxidativo e fluxo de lípides em macrófagos: papel dos inibidores de glicação avançada
Beneficiário:Raphael de Souza Pinto
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 07/59710-6 - Glicação avançada em macrófagos diminui o conteúdo dos receptores de HDL - ABCA-1 e ABCG-1- e induz acúmulo intracelular de 7 -cetocolesterol
Beneficiário:Rodrigo Tallada Iborra
Linha de fomento: Bolsas no Brasil - Doutorado