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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Oligomerization, Membrane Association, and in Vivo Phosphorylation of Sugarcane UDP-glucose Pyrophosphorylase

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Autor(es):
Soares, Jose Sergio M. [1] ; Gentile, Agustina [1] ; Scorsato, Valeria [2] ; Lima, Aline da C. [2] ; Kiyota, Eduardo [2] ; dos Santos, Marcelo Leite [3] ; Piattoni, Claudia V. [4] ; Huber, Steven C. [5, 6] ; Aparicio, Ricardo [2] ; Menossi, Marcelo [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, BR-6109 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Quim, Lab Biol Estrutural & Cristalog, BR-6154 Campinas, SP - Brazil
[3] Univ Fed Sergipe, Nucleo Quim, Ctr Ciencias Exatas & Tecnol, BR-49500000 Itabaiana, SE - Brazil
[4] Univ Nacl Litoral, Inst Agrobiotecnol Litoral UNL CONICET, Santa Fe - Argentina
[5] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 - USA
[6] Univ Illinois, Dept Agr Agr Res Serv, Urbana, IL 61801 - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Journal of Biological Chemistry; v. 289, n. 48, p. 33364-33377, NOV 28 2014.
Citações Web of Science: 6
Resumo

Sugarcane is a monocot plant that accumulates sucrose to levels of up to 50% of dry weight in the stalk. The mechanisms that are involved in sucrose accumulation in sugarcane are not well understood, and little is known with regard to factors that control the extent of sucrose storage in the stalks. UDPglucose pyrophosphorylase (UGPase; EC 2.7.7.9) is an enzyme that produces UDP-glucose, a key precursor for sucrose metabolism and cell wall biosynthesis. The objective of this work was to gain insights into the ScUGPase-1 expression pattern and regulatory mechanisms that control protein activity. ScUGPase-1 expression was negatively correlated with the sucrose content in the internodes during development, and only slight differences in the expression patterns were observed between two cultivars that differ in sucrose content. The intracellular localization of ScUGPase-1 indicated partial membrane association of this soluble protein in both the leaves and internodes. Using a phospho-specific antibody, we observed that ScUGPase-1 was phosphorylated in vivo at the Ser-419 site in the soluble and membrane fractions from the leaves but not from the internodes. The purified recombinant enzyme was kinetically characterized in the direction of UDP-glucose formation, and the enzyme activity was affected by redox modification. Preincubation with H2O2 strongly inhibited this activity, which could be reversed by DTT. Small angle x-ray scattering analysis indicated that the dimer interface is located at the C terminus and provided the first structural model of the dimer of sugarcane UGPase in solution. (AU)

Processo FAPESP: 08/06767-3 - Caracterização estrutural e funcional de enzimas envolvidas na biossíntese e acúmulo de sacarose em cana-de-açúcar
Beneficiário:José Sérgio de Macedo Soares
Linha de fomento: Bolsas no Brasil - Doutorado